Cat. #151412
Anti-p53 [Pab 246]
Cat. #: 151412
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: p53 mouse, wild type only
Class: Monoclonal
Application: IHC ; IP
Reactivity: Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: David Lane
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Primary Citation: Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-p53 [Pab 246]
- Clone: Pab 246
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 50 kDa
- Strain: Balb/c
- Reactivity: Mouse
- Host: Mouse
- Application: IHC ; IP
- Description: Anti-p53 clone Pab 246 recognises the tumour antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is upregulated in response to DNA damage and is found in a wide variety of transformed cells. Mutations in p53 can lead to conformational changes in the protein, exposing different epitopes. Pab 246 binds to the wild-type conformation of p53
- Immunogen: SV40-transformed cell line Balb/c SVA31 E7.
- Immunogen uniprot id: P02340
- Isotype: IgG1 kappa
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: p53 mouse, wild type only
- Molecular weight: 50 kDa
- Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations.
Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of fun...
Applications
- Application: IHC ; IP
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Pradhan et al. 2019. Nucleic Acids Res. 47(4):1637-1652. PMID: 30649466.
- Hwang et al. 2018. Cell Rep. 22(1):299-312. PMID: 29298430.
- Labalette et al. 2008. PLoS One. 3(11):e3761. PMID: 19018287.
- Han et al. 2008. Cell Stem Cell. 2(3):241-51. PMID: 18371449.
- Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.
- Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.
- Wade-Evans et al. 1985. EMBO J. 4(3):699-706. PMID: 2408882.
