- Reviews (1)
|Applications||ChIP ELISA IHC WB|
|Antigen/Gene or Protein Targets||TAL1|
|Relevance||T-cell acute lymphocytic leukemia protein 1 (TAL1) is a basic helix-loop-helix transcription factor implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, including a positive regulation of erythroid differentiation. Diseases associated with TAL1 include leukemia, acute lymphoblastic and precursor t-cell acute lymphoblastic leukemia. A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL).|
|Immunogen||TAL-1 recombinant protein|
|Recommended Growing Conditions||It is advised that cells are grown in the following medium:RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.|
|Research Area||Cancer, Cell Cycle, Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology|
References: 5 entries
Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.
Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.
Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.
Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.
Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.
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- WB (1)
Have you used this reagent? Share your experience by submitting a review and help your fellow scientists.Review this reagent
|Tissue / cell type||T-acute lymphoblastic leukemia (T-ALL) cell lines|
Nuclear extracts were resolved on 4-12% Bolt Bis-Tris Acrylamide gel (Invitrogen), transferred to nitrocellulose membrane and blocked with 5% low-fat milk powder (Marvel) in PBS. Primary antibody was used 1:1000 in PBS, followed by incubation with the secondary IRDye® 800CW Donkey anti-Mouse IgG (LiCor). Fluorescence was detected using Odyssey imaging system (LI-COR)
Review provided by Dr Vesna Stanulovic with data from Hoogenkamp lab mambers, University of Birmingham