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Anti-TAL1 [BTL73]

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151415
Applications ChIP ELISA IHC WB
Antigen/Gene or Protein Targets TAL1
Reactivity Human
Relevance Monoclonal antibody which stains 3 forms of TAL1 in the cytoplasm and nuclei of cells.

Background and Research Application
T-cell acute lymphocytic leukaemia protein 1 (TAL1) is a basic helix-loop-helix transcription factor implicated in the genesis of hemopoietic malignancies. TAL1 also known as SCL (stem cell leukaemia), is a serine phosphoprotein and basic-helix-loop-helix transcription factor. It may play an important role in hemopoietic differentiation, including a positive regulation of erythroid differentiation.
Diseases associated with TAL1 include leukaemia, acute lymphoblastic and precursor T-cell acute lymphoblastic leukaemia. A non-random site-specific chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukaemia’s (T-ALL). This results in the aberrant expression of TAL1 mRNA in the neoplastic cells.
Anti-TAL1 BTL73 recognises and immunoprecipitates all 3 forms of the TAL1 protein, at 22,39, and 41 kD. It is likely this antibody binds an epitope at the carboxyl terminus of the TAL1 protein.
Host Mouse
Immunogen TAL-1 recombinant protein
Immunogen UniProt ID P17542
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions It is advised that cells are grown in the following medium:RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody stains the cytoplasm and nuclei of DU.528 cells.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Cycle, Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology

References

There are 11 reference entries for this reagent.

View All References

References: 11 entries

Christophersen et al. 2017. Sci Rep. 7:40451. PMID: 28084402.

Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.

A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.

Europe PMC ID: 21385855

Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.

Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.

Europe PMC ID: 12091340

Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.

TAL1 expression does not occur in the majority of T-ALL blasts.

Europe PMC ID: 9695959

Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.

Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.

Europe PMC ID: 7756670

Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.

Expression of TAL-1 proteins in human tissues.

Europe PMC ID: 7833471


Add a reference

References: 11 entries

Christophersen et al. 2017. Sci Rep. 7:40451. PMID: 28084402.

Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.

A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.

Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.

Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.

Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.

TAL1 expression does not occur in the majority of T-ALL blasts.

Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.

Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.

Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.

Expression of TAL-1 proteins in human tissues.


Add a reference