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Anti-TAL1 [BTL73]


Catalogue Number 151415
Applications ChIP ELISA IHC WB
Antigen/Gene or Protein Targets TAL1
Reactivity Human
Relevance T-cell acute lymphocytic leukemia protein 1 (TAL1) is a basic helix-loop-helix transcription factor implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, including a positive regulation of erythroid differentiation. Diseases associated with TAL1 include leukemia, acute lymphoblastic and precursor t-cell acute lymphoblastic leukemia. A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL).
Host Mouse
Immunogen TAL-1 recombinant protein
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions It is advised that cells are grown in the following medium:RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Cycle, Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology

References: 5 entries

Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.

Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.

Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.

Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.

Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.

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Application Western Blot
Reactivity Human
Tissue / cell type T-acute lymphoblastic leukemia (T-ALL) cell lines

Nuclear extracts were resolved on 4-12% Bolt Bis-Tris Acrylamide gel (Invitrogen), transferred to nitrocellulose membrane and blocked with 5% low-fat milk powder (Marvel) in PBS. Primary antibody was used 1:1000 in PBS, followed by incubation with the secondary IRDye® 800CW Donkey anti-Mouse IgG (LiCor). Fluorescence was detected using Odyssey imaging system (LI-COR)

Additional notes

Review provided by Dr Vesna Stanulovic with data from Hoogenkamp lab mambers, University of Birmingham