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Anti-TAL1 [BTL73]

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151415
Applications ChIP ELISA IHC WB
Antigen/Gene or Protein Targets TAL1
Reactivity Human
Relevance T-cell acute lymphocytic leukemia protein 1 (TAL1) is a basic helix-loop-helix transcription factor implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, including a positive regulation of erythroid differentiation. Diseases associated with TAL1 include leukemia, acute lymphoblastic and precursor t-cell acute lymphoblastic leukemia. A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL).
Host Mouse
Immunogen TAL-1 recombinant protein
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions It is advised that cells are grown in the following medium:RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Cycle, Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology

References: 10 entries

Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.

A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.

Europe PMC ID: 21385855

Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.

Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.

Europe PMC ID: 12091340

Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.

TAL1 expression does not occur in the majority of T-ALL blasts.

Europe PMC ID: 9695959

Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.

Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.

Europe PMC ID: 7756670

Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.

Expression of TAL-1 proteins in human tissues.

Europe PMC ID: 7833471


Add a reference

References: 10 entries

Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.

A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.

Europe PMC ID: 21385855

Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.

Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.

Europe PMC ID: 12091340

Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.

TAL1 expression does not occur in the majority of T-ALL blasts.

Europe PMC ID: 9695959

Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.

Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.

Europe PMC ID: 7756670

Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.

Expression of TAL-1 proteins in human tissues.

Europe PMC ID: 7833471


Add a reference

References: 10 entries

Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.

A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.

Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.

Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.

Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.

TAL1 expression does not occur in the majority of T-ALL blasts.

Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.

Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.

Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.

Expression of TAL-1 proteins in human tissues.


Add a reference
Rating
Application Western Blot
Reactivity Human
Tissue / cell type T-acute lymphoblastic leukemia (T-ALL) cell lines
Method

Nuclear extracts were resolved on 4-12% Bolt Bis-Tris Acrylamide gel (Invitrogen), transferred to nitrocellulose membrane and blocked with 5% low-fat milk powder (Marvel) in PBS. Primary antibody was used 1:1000 in PBS, followed by incubation with the secondary IRDye® 800CW Donkey anti-Mouse IgG (LiCor). Fluorescence was detected using Odyssey imaging system (LI-COR)


Additional notes

Review provided by Dr Vesna Stanulovic with data from Hoogenkamp lab mambers, University of Birmingham