Anti-TAL1 [BTL73]
Invented by Karen Pulford from University of Oxford
Invented at University of Oxford
- Datasheet
- References (11)
- Inventor Info
Info
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| Catalogue Number | 151415 |
| Applications | ChIP ELISA IHC WB |
| Antigen/Gene or Protein Targets | TAL1 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which stains 3 forms of TAL1 in the cytoplasm and nuclei of cells. Background and Research Application T-cell acute lymphocytic leukaemia protein 1 (TAL1) is a basic helix-loop-helix transcription factor implicated in the genesis of hemopoietic malignancies. TAL1 also known as SCL (stem cell leukaemia), is a serine phosphoprotein and basic-helix-loop-helix transcription factor. It may play an important role in hemopoietic differentiation, including a positive regulation of erythroid differentiation. Diseases associated with TAL1 include leukaemia, acute lymphoblastic and precursor T-cell acute lymphoblastic leukaemia. A non-random site-specific chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukaemia’s (T-ALL). This results in the aberrant expression of TAL1 mRNA in the neoplastic cells. Anti-TAL1 BTL73 recognises and immunoprecipitates all 3 forms of the TAL1 protein, at 22,39, and 41 kD. It is likely this antibody binds an epitope at the carboxyl terminus of the TAL1 protein. |
| Host | Mouse |
| Immunogen | TAL-1 recombinant protein |
| Immunogen UniProt ID | P17542 |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | It is advised that cells are grown in the following medium:RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well. |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody stains the cytoplasm and nuclei of DU.528 cells. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Cycle, Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology |
References: 11 entries
Christophersen et al. 2017. Sci Rep. 7:40451. PMID: 28084402.
Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.
A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.
Europe PMC ID: 21385855
Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.
Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.
Europe PMC ID: 12091340
Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.
TAL1 expression does not occur in the majority of T-ALL blasts.
Europe PMC ID: 9695959
Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.
Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.
Europe PMC ID: 7756670
Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.
Expression of TAL-1 proteins in human tissues.
Europe PMC ID: 7833471
Add a reference
References: 11 entries
Christophersen et al. 2017. Sci Rep. 7:40451. PMID: 28084402.
Farrell et al. 2011. Blood. 117(18):4935-45. PMID: 21385855.
A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression.
Goardon et al. 2002. Blood. 100(2):491-500. PMID: 12091340.
Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation.
Delabesse et al. 1998. Br J Haematol. 102(2):449-57. PMID: 9695959.
TAL1 expression does not occur in the majority of T-ALL blasts.
Bernard et al. 1995. Blood. 85(11):3356-7. PMID: 7756670.
Nuclear localization of the SCL/TAL1 basic helix-loop-helix protein is not dependent on the presence of the basic domain.
Pulford et al. 1995. Blood. 85(3):675-84. PMID: 7833471.
Expression of TAL-1 proteins in human tissues.
Add a reference
