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Krt5-Cre;Braff/f;Raf1f/f Mouse

Invented by Prof Manuela Baccarini at University of Vienna

Info

Catalogue Number 152988
Antigen/Gene or Protein Targets Epidermis-specific double knockout of Braf and Raf1 alleles
Disease Keywords Atopic Dermatitis
Synonyms RAF, CRAF, BRAF1, RAFB1, B-Raf, RAF/MEK/ERK signalling pathway, Cre-loxP recombination, Eczema
Model Transgenic
Relevance The skin is the largest organ of the body protecting it from damage and hosting immune system cells. To function effectively as a barrier, the skin continually regenerates producing new cells to replace the old. If the regeneration process fails the skin may produce tumours, and disruption to the immune barrier can lead to autoimmune or allergic skin diseases such as psoriasis or atopic dermatitis.

This mouse model has epidermis-specific Braf/Raf1 ablation, mediated by Cre expression in the basal epidermal layer and follicular keratinocytes using the Keratin 5 promoter. In normal conditions Braf and RAF1 operate independently to balance MAPK signalling. Removal of Braf and Raf1 in this model caused mice to develop an allergic disease similar to human atopic dermatitis, characterized by IgE responses (chemokines and Th2-type cytokines) with local and systemic inflammation.
Production Details Krt5-Cre;Braff/f;Raf1f/f mice were generated by crossing Krt5-Cre;Braff/f with Krt5-Cre;Raf1f/f animals
Conditional Yes
Conditional Description Tissue-specific Cre expression driven by the human Keratin 5 promoter ablating both Braf and Raf1 alleles in the epidermis
Mouse Genetic Background/Cross History Mouse strain: 129S1/SvImJ also known as 129S1
Zygosity Homozygous for both floxed Braf and floxed Raf1 alleles
Research Area Bacteriology, Developmental Biology, Drug Discovery & Development, Genetic Studies Tools

References

There are 6 reference entries for this reagent.

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References: 6 entries

Raguz et al. 2016. Elife. 5:. PMID: 27431613.

Epidermal RAF prevents allergic skin disease.

Europe PMC ID: 27431613

Kern et al. 2013. Oncogene. 32(19):2483-92. PMID: 22733131.

Essential, non-redundant roles of B-Raf and Raf-1 in Ras-driven skin tumorigenesis.

Europe PMC ID: 22733131

Ehrenreiter et al. 2005. J Cell Biol. 168(6):955-64. PMID: 15753127.

Raf-1 regulates Rho signaling and cell migration.

Europe PMC ID: 15753127


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References: 6 entries

Raguz et al. 2016. Elife. 5:. PMID: 27431613.

Epidermal RAF prevents allergic skin disease.

Kern et al. 2013. Oncogene. 32(19):2483-92. PMID: 22733131.

Essential, non-redundant roles of B-Raf and Raf-1 in Ras-driven skin tumorigenesis.

Ehrenreiter et al. 2005. J Cell Biol. 168(6):955-64. PMID: 15753127.

Raf-1 regulates Rho signaling and cell migration.


Add a reference