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Anti-RNA polII [CTD 4H8]


Catalogue Number 151247
Applications ChIP ELISA FACS IHC IF IP WB ChIP-seq
Antigen/Gene or Protein Targets RNA Polymerase II
Reactivity Human and Saccharomyces cerevisiae
Relevance RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
Host Mouse
Immunogen Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Subclass IgG1
Positive Control HeLa cell line.
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein.
Research Area Epigenetics & Nuclear Signalling

References: 10 entries

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Seo et al. 2014. Nucleic Acids Res. 42(2):1016-25. PMID: 24163101.

Keren-Shaul et al. 2013. PLoS One. 8(1):e53506. PMID: 23326444.

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Li et al. 2006. Mol Biol Cell. 17(9):3848-59. PMID: 16807355.

Yart et al. 2005. Mol Cell Biol. 25(12):5052-60. PMID: 15923622.

Engelhardt et al. 2005. J Virol. 79(9):5812-8. PMID: 15827195.

Kristjuhan et al. 2003. Proc Natl Acad Sci U S A. 100(13):7551-6. PMID: 12796514.

Kristjuhan et al. 2002. Mol Cell. 10(4):925-33. PMID: 12419235.

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