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Catalogue Number 152722
Backbone Size (bp) 5169
Bacterial Resistance Ampicillin
Vector Type pBabe-puro
Antigen/Gene or Protein Targets Rock1, Rock
Relevance Rock1:ER-KD Plasmid contains a kinase-dead human ROCK I (2–537) in which Lys105 is mutated to glycine. ROCK I KD is fused to the hormone-binding domain of the estrogen receptor. Rock1:ER-KD is the control for Rock1:ER (catalogue code 152721). The KD mutant was applied in Croft et al., 2005.The protein is fused to eGFP for visualisation.

Ultimately, Rock1 activation leads to phosphorylation of proteins involved in the regulation of the actin‐myosin cytoskeleton, generation of pronounced actin stress fibers, and changes in cell morphology. Rock1:ER GFP serves as a good complement for small‐molecule Rock1 inhibitors.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility

References

There are 2 reference entries for this reagent.

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References: 2 entries

Croft et al. 2005. J Cell Biol. 168(2):245-55. PMID: 15657395.

Actin-myosin-based contraction is responsible for apoptotic nuclear disintegration.

Europe PMC ID: 15657395


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References: 2 entries

Croft et al. 2005. J Cell Biol. 168(2):245-55. PMID: 15657395.

Actin-myosin-based contraction is responsible for apoptotic nuclear disintegration.


Add a reference