Cancer Research Technology
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Catalogue Number 152609
Antigen/Gene or Protein Targets ULK1, ULK2
Host Mouse
Tissue Embryo
Disease Keywords Cancer, neurodegeneration, infectious disease
Model Knock-Out
Relevance The MEF ULK1 KO (SV40) cell line provides a tool for the study of Ulk1 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk2 KO cell lines. This cell line is derived from an embryo with a mixed genetic background (Blk6/129 Agouti) and has been immortalised with SV40 large T-antigen.
Production Details Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 +/+ and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol.
Research Area Apoptosis and Programmed Cell Death, Cell Signaling & Signal Transduction, Metabolism, Neurobiology
Growth/Phenotype Keywords Fibroblast
Recommended Growing Conditions DMEM + 20% FCS + 2mM Glutamine + pen/strep
Cellosaurus ID CVCL_5A57

References

There are 2 reference entries for this reagent.

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References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Europe PMC ID: 23291478


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References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.


Add a reference