MEF ULK1/2 WT (SIM) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
MEF ULK1 ULK2 DKO (SV40) Cell Line
Invented by Dr Sharon Tooze from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 152608 |
| Antigen/Gene or Protein Targets | ULK1, ULK2 |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer, neurodegeneration, infectious disease |
| Model | Knock-Out |
| Relevance | The MEF ULK1 ULK2 DKO (SV40) cell line provides a tool for the study of Ulk1 and Ulk2 and of Autophagy. Mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti). Cells were immortalized with SV40 large T-antigen |
| Production Details | Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol. |
| Research Area | Apoptosis and Programmed Cell Death, Cell Signaling & Signal Transduction, Metabolism, Neurobiology |
| Growth/Phenotype Keywords | Fibroblast |
| Recommended Growing Conditions | DMEM + 20% FCS + 2mM Glutamine + pen/strep |
| Cellosaurus ID | CVCL_5A57 |
MEF ULK1 KO (SIM) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
MEF ULK1/2 WT(SV40) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
MEF ULK2 KO (SIM) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
MEF ULK1 ULK2 DKO (SIM) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
MEF ULK1 KO (SV40) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
MEF ULK2 KO (SV40) Cell Line
Invented by Dr Sharon Tooze at Cancer Research UK London Research Institute: Lincoln's Inn Fields
References: 2 entries
McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
Europe PMC ID: 23291478
Add a reference
References: 2 entries
McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
Add a reference
