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MEF ULK1 ULK2 DKO (SIM) Cell Line

Invented by Dr Sharon Tooze at The Francis Crick Institute

Info

Catalogue Number 151774
Antigen/Gene or Protein Targets ULK1, ULK2
Host Mouse
Tissue Embryo
Disease Keywords Cancer, neurodegeneration, infectious disease
Model Knock-Out
Relevance The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of Autophagy. Mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti).
Production Details Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SIM using a standard serial passaging protocol.
Research Area Apoptosis and Programmed Cell Death, Cell Signaling & Signal Transduction, Metabolism, Neurobiology
Growth/Phenotype Keywords Fibroblast
Recommended Growing Conditions DMEM + 20% FCS + 2mM Glutamine + pen/strep
Cellosaurus ID CVCL_5A56

References: 1 entry

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.


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References: 1 entry

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.


Add a reference

References: 1 entry

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.


Add a reference


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