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MEF ULK1 KO (SIM) Cell Line

Invented by Dr Sharon Tooze at The Francis Crick Institute

Info

Catalogue Number 151715
Antigen/Gene or Protein Targets ULK1
Host Mouse
Tissue Embryo
Disease Keywords Cancer, neurodegeneration, infectious disease
Model Knock-Out
Relevance The MEF ULK1 KO (SIM) cell line can be used to study ULK1-dependent processes, including autophagy. A more complete phenotype requires depletion of ULK2 by RNAi . Mouse embryonic fibroblasts derived from a ULK1 homozygous knock out mouse embryo and immortalized by serial passaging (spontaneous transformation).
Production Details Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- mouse at day 13p.c. The MEFs were immortalised by SIM using a standard serial passaging protocol.
Research Area Apoptosis and Programmed Cell Death, Cell Signaling & Signal Transduction, Metabolism, Neurobiology
Growth/Phenotype Keywords Autophagy, fibroblast
Recommended Growing Conditions DMEM + 20% FCS + 2mM Glutamine + pen/strep
Cellosaurus ID CVCL_5A52

References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.


Add a reference

References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.


Add a reference

References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.


Add a reference


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