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Catalogue Number 152607
Antigen/Gene or Protein Targets ULK1
Host Mouse
Tissue Embryo
Disease Keywords Cancer, neurodegeneration
Relevance The MEF ULK1/2 WT cell line can be used as a wild-type control with ULK1 and ULK2 cell lines and is useful for studying ULK1-and ULK-2 dependent processes, including autophagy. Mouse embryonic fibroblasts derived from a wild-type littermate to the ULK1 KO and immortalized with SV40 large T-antigen.
Production Details Cells were derived from mouse embryos at E13.5. Embryos were decapitated and eviscerated and the remaining tissues were dissociated to obtain MEFs. This cell line is a primary cell line immortalized by SV40.
Research Area Apoptosis and Programmed Cell Death, Cell Signaling & Signal Transduction, Metabolism, Neurobiology
Growth/Phenotype Keywords Autophagy
Recommended Growing Conditions DMEM + 20% FCS + 2mM Glutamine + pen/strep
Cellosaurus ID CVCL_5A51

References

There are 4 reference entries for this reagent.

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References: 4 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Europe PMC ID: 23291478

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.

Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.

Europe PMC ID: 18936157


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References: 4 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.

Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.


Add a reference