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Cell Lines

KARPAS 1718 Cell Line

Invented by Abraham Karpas
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KARPAS 1718 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
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Antigen/Gene or Protein Targets Human splenic lymphoma
Host Human
Tissue Blood
Disease Keywords Cancer; haemotology; lymphoma
Relevance The KARPAS 1718 cell line is derived from transformed splenic lymphoma with villous lymphocytes; useful in oncology research.
Production Details Derived from the peripheral blood of a patient diagnosed with transformed splenic lymphoma with villous lymphocytes.
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Single round to polymorphic cells growing in suspension
Recommended Growing Conditions Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 105 cells/ml, 5% CO2, 37°c. For cryopreservation of KARPAS 1718, freeze at approximately 2 x 107 cells/ml in 90% FBS + 10% DMSO.Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Cellosaurus ID CVCL_2539

References

There are 3 reference entries for this reagent.

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References: 3 entries

Martinez-Climent et al. 2003. Leuk Lymphoma. 44(3):459-64. PMID: 12688315.

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Ota et al. 2004. Cancer Res. 64(9):3087-95. PMID: 15126345.

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Sonoki et al. 2004. Leukemia. 18(12):2026-31. PMID: 15496980.

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References: 3 entries

Martinez-Climent et al. 2003. Leuk Lymphoma. 44(3):459-64. PMID: 12688315.

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Ota et al. 2004. Cancer Res. 64(9):3087-95. PMID: 15126345.

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Sonoki et al. 2004. Leukemia. 18(12):2026-31. PMID: 15496980.

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Inventor Information