KARPAS 1718 Cell Line
Invented by Abraham Karpas at University of Cambridge
- Datasheet
- References (3)
- Inventor Info
Info
| Antigen/Gene or Protein Targets | Human splenic lymphoma |
| Host | Human |
| Tissue | Blood |
| Disease Keywords | Cancer; haemotology; lymphoma |
| Relevance | The KARPAS 1718 cell line is derived from transformed splenic lymphoma with villous lymphocytes; useful in oncology research. |
| Production Details | Derived from the peripheral blood of a patient diagnosed with transformed splenic lymphoma with villous lymphocytes. |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Single round to polymorphic cells growing in suspension |
| Recommended Growing Conditions | Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 105 cells/ml, 5% CO2, 37°c. For cryopreservation of KARPAS 1718, freeze at approximately 2 x 107 cells/ml in 90% FBS + 10% DMSO.Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Cellosaurus ID | CVCL_2539 |
References: 3 entries
Martinez-Climent et al. 2003. Leuk Lymphoma. 44(3):459-64. PMID: 12688315.
Ota et al. 2004. Cancer Res. 64(9):3087-95. PMID: 15126345.
Sonoki et al. 2004. Leukemia. 18(12):2026-31. PMID: 15496980.
Add a reference
References: 3 entries
Martinez-Climent et al. 2003. Leuk Lymphoma. 44(3):459-64. PMID: 12688315.
Ota et al. 2004. Cancer Res. 64(9):3087-95. PMID: 15126345.
Sonoki et al. 2004. Leukemia. 18(12):2026-31. PMID: 15496980.
Add a reference
Inventor Information
Inventors
|
Abraham Karpas |
