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|Applications||ELISA IF WB|
|Antigen/Gene or Protein Targets||GSK-3 (phospho-Ser9)|
|Relevance||The Monoclonal Anti-GSK-3 / Shaggy protein kinase family, Clone 7G-2G5 specifically reacts with the peptide when phosphorylated on ser9, and detects selectively the corresponding phospho-isoform of the GSK-3/Shaggy enzyme of different origins (rat, mouse, human...) that are present in cellular extrats. The phosphorylated peptide used as an immunogen derives from the N-terminal of the shaggy polypeptide and is well conserved in comparable regions of the rat GSK-3- alpha and beta enzymes (position 8 is a T in GSK-3-beta, and position 5 is an A in GSK-3- alpha). Both insulin and EGF treatments of cells induce increased reactivity to this antibody as the result of an increased phosphorylation. This phosphorylation is associated with reduced activity of the enzyme. Glycogen synthase kinase 3 is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues. Phosphorylation of a protein by GSK-3 usually inhibits the activity of its downstream target. GSK-3 is active in a number of central intracellular signaling pathways, including cellular proliferation, migration, glucose regulation, and apoptosis.|
Available in 100ul unit as ascites.
Recommended dilutions : 1/500 - 1/5000
|Research Area||Apoptosis and Programmed Cell Death, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism|
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