- References (2)
- Inventor Info
|Applications||ELISA IF WB|
|Antigen/Gene or Protein Targets||Gal-4 (DNA Binding domain)|
|Relevance||The Monoclonal Anti-Gal 4 (DNA Binding Domain), clone 3GV-2 recognises DBD containing proteins and has been validated for ELISA, Immunoblotting, Transcription assays, immunocytochemistry on transfected cells and it inhibits at 70% DNA binding of Gal 4 DBD. It is not suitable for immunoprecipitation. GAL4 is a modular protein consisting broadly of a DNA-binding domain and an activation domain. Although GAL4 is a yeast protein not normally present in other organisms it has been shown to work as a transcription activator in a variety of organisms such as Drosophila, and human cells, highlighting that the same mechanisms for gene expression have been conserved over the course of evolution. Together with UAS (Upstream Activation Sequence), an enhancer to which GAL4 specifically binds to activate gene transcription, Gal 4 forms The GAL4-UAS system, a biochemical method used to study gene expression and function in different organisms.|
|Immunogen||Peptide of Gal 4 DBD coupled to ovalbumin : 138 N‐K‐G‐Q‐R‐Q‐L‐T‐V‐S147‐(C).|
Available in 100ul unit as ascites.
Recommended dilutions : 1/1000-1/5000
|Research Area||Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Epitope Tags, Metabolism|
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