Anti-G-quadruplex, Recombinant [BG4]
Invented by Shankar Balasubramanian , John McCafferty , David Tannahill , Giulia Biffi
Invented at University of Cambridge
- Datasheet
- References (8)
- Inventor Info
Info
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| Applications | ELISA IF ChIP-seq |
| Antigen/Gene or Protein Targets | DNA/RNA G-quadruplex structures in human cells |
| Reactivity | Human |
| Relevance |
BG4 is an engineered, structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA G-quadruplex structures can can be employed to visualize DNA G-quadruplex structures in the genomic DNA of human cells. BG4 has high affinity for intramolecular and intermolecular DNA G-quadruplexes (Kd 0.5 – 1.6 nM, and 2.0 nM respectively) with no detectable binding to a RNA hairpin, single-stranded or double-stranded DNA. |
| Immunogen | BG4, a single-chain antibody, was isolated from the Sanger phage display library through selection using a panel of intramolecular G-quadruplex structures. |
| Research Area | Epigenetics & Nuclear Signalling |
References: 8 entries
Biffi et al. 2013. Nat Chem. 5(3):182-6. PMID: 23422559.
IF ELISA
Biffi et al. 2014. Nat Chem. 6(1):75-80. PMID: 24345950.
IF
Yangyuoru et al. 2015. Angew Chem Weinheim Bergstr Ger. 127(3):924-927. PMID: 26300569.
Hänsel-Hertsch et al. 2016. Nat Genet. :. PMID: 27618450.
ChIP-seq
G-quadruplex structures mark human regulatory chromatin.
Europe PMC ID: 27618450
Dual Binding of an Antibody and a Small Molecule Increases the Stability of TERRA G-Quadruplex.
Europe PMC ID: 26300569
Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells.
Europe PMC ID: 24345950
Quantitative visualization of DNA G-quadruplex structures in human cells.
Europe PMC ID: 23422559
Add a reference
References: 8 entries
Biffi et al. 2013. Nat Chem. 5(3):182-6. PMID: 23422559.
IF ELISA
Biffi et al. 2014. Nat Chem. 6(1):75-80. PMID: 24345950.
IF
Yangyuoru et al. 2015. Angew Chem Weinheim Bergstr Ger. 127(3):924-927. PMID: 26300569.
Hänsel-Hertsch et al. 2016. Nat Genet. :. PMID: 27618450.
ChIP-seq
G-quadruplex structures mark human regulatory chromatin.
Dual Binding of an Antibody and a Small Molecule Increases the Stability of TERRA G-Quadruplex.
Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells.
Quantitative visualization of DNA G-quadruplex structures in human cells.
Add a reference
Inventor Information
Inventors
|
Shankar Balasubramanian |
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|
John McCafferty |
|
|
David Tannahill |
|
|
Giulia Biffi |
