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Anti-G-quadruplex, Recombinant [BG4]

Invented by Shankar Balasubramanian, at University of Cambridge John McCafferty, at University of Cambridge David Tannahill, at University of Cambridge Giulia Biffi at University of Cambridge

Info

Applications ELISA IF ChIP-seq
Antigen/Gene or Protein Targets DNA/RNA G-quadruplex structures in human cells
Reactivity Human
Relevance BG4 is an engineered, structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA G-quadruplex structures can can be employed to visualize DNA G-quadruplex structures in the genomic DNA of human cells.
BG4 has high affinity for intramolecular and intermolecular DNA G-quadruplexes (Kd 0.5 – 1.6 nM, and 2.0 nM respectively) with no detectable binding to a RNA hairpin, single-stranded or double-stranded DNA.
Immunogen BG4, a single-chain antibody, was isolated from the Sanger phage display library through selection using a panel of intramolecular G-quadruplex structures.
Research Area Epigenetics & Nuclear Signalling

References

There are 8 reference entries for this reagent.

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References: 8 entries

Biffi et al. 2013. Nat Chem. 5(3):182-6. PMID: 23422559.

IF ELISA

Biffi et al. 2014. Nat Chem. 6(1):75-80. PMID: 24345950.

IF

Yangyuoru et al. 2015. Angew Chem Weinheim Bergstr Ger. 127(3):924-927. PMID: 26300569.

Hänsel-Hertsch et al. 2016. Nat Genet. :. PMID: 27618450.

ChIP-seq

G-quadruplex structures mark human regulatory chromatin.

Europe PMC ID: 27618450

Dual Binding of an Antibody and a Small Molecule Increases the Stability of TERRA G-Quadruplex.

Europe PMC ID: 26300569

Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells.

Europe PMC ID: 24345950

Quantitative visualization of DNA G-quadruplex structures in human cells.

Europe PMC ID: 23422559


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References: 8 entries

Biffi et al. 2013. Nat Chem. 5(3):182-6. PMID: 23422559.

IF ELISA

Biffi et al. 2014. Nat Chem. 6(1):75-80. PMID: 24345950.

IF

Yangyuoru et al. 2015. Angew Chem Weinheim Bergstr Ger. 127(3):924-927. PMID: 26300569.

Hänsel-Hertsch et al. 2016. Nat Genet. :. PMID: 27618450.

ChIP-seq

G-quadruplex structures mark human regulatory chromatin.

Dual Binding of an Antibody and a Small Molecule Increases the Stability of TERRA G-Quadruplex.

Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells.

Quantitative visualization of DNA G-quadruplex structures in human cells.


Add a reference

Inventor Information

Inventors

Shankar Balasubramanian

Shankar Balasubramanian

John McCafferty

John McCafferty

David Tannahill

David Tannahill

Giulia Biffi

Giulia Biffi

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