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Proximal Tubular Epithelial Cell Line (ciPTEC)

Invented by Prof Moin Saleem at University Of Bristol

Info

Host Human
Tissue Kidney
Model Primary line
Relevance The Proximal Tubular Epithelial Cell Line (ciPTEC) is a primary cell line isolated from human kidneys. Proximal tubular cells (PTCs) are vital for the reabsorption of filtered solutes from the glomerular filtrate of the kidney. Key to this function is the expression of various transporters. Current available human immortalised PTC lines express too few transporters, and primary cells offer a limited amount of material before dedifferentiation occurs. Conditional immortalisation of the PTC line overcomes these issues, by enabling the in-vitro differentiation of cells in a manner similar to in-vivo differentiation. Unlike other cell models, this homogenous, stable cell line expresses two of the key transporters (apical efflux transporter p-glycoprotein and basolateral influx transporter organic cation transporter 2) pivotal to the PTC functionality.
Conditional Description Immortalised with SV40T and hTERT vectors containing respectively geneticin (G418) or hygromycin resistance
Research Area Cellular Transport
Recommended Growing Conditions RPMI 1640 medium supplemented with 10% fetal calf serum, 10 U/ml streptomycin, 10 U/ml penicillin and ITS
Notes Applications:
Toxicology studies
Metabolism
Therapeutic targeting
Renal physiology R&D
Co-culture studies (e.g 3D culture model)

Key benefits:
Primary human cell derived
Physiologically relevant phenotype analogous to in-vivo differentiated renal cells
Homogenous, stable and long lasting
Cellosaurus ID CVCL_W184

References: 1 entry

Wilmer et al. 2010. Cell Tissue Res. 339(2):449-57. PMID: 19902259.


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References: 1 entry

Wilmer et al. 2010. Cell Tissue Res. 339(2):449-57. PMID: 19902259.


Add a reference

References: 1 entry

Wilmer et al. 2010. Cell Tissue Res. 339(2):449-57. PMID: 19902259.


Add a reference


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