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Human Podocyte Cell Line (CIHP-1)

Invented by Prof Moin Saleem at University Of Bristol

Info

Host Human
Tissue Kidney
Model Primary line
Relevance CIHP-1 cell line to study the maturation of podocytes in-vivo.

Background and Research Application
The Human Podocyte Cell Line (CIHP-1) is a primary cell line isolated from human kidney. Podocytes are highly specialised, terminally differentiated cells with a complex cellular structure. They are a critical component of glomerular filtration. Cell cycle control, growth arrest and differentiation are key to the in-vivo biology of podocytes. The conditionally immortalised podocyte cell line allows an in-vitro process of maturation analogous to the development and maturation of podocytes in-vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte proteins nephrin, podocin, CD2AP and synaptopodin. This robust cell line is extensively characterised and validated, used worldwide with over 100 peer reviewed publications to date.
Conditional Description Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT
Research Area Other
Recommended Growing Conditions RPMI-1640, Insulin-Transferrin-Selenium, Foetal Bovine Serum (10% v/v), Pen/Strep medium at at 33°C or 37°C, 5% CO2.
Notes Points of Interest
Applications:
Toxicology studies
Metabolism
Therapeutic targeting
Renal physiology R&D
Co-culture studies (e.g 3D culture model)

Key benefits:
Primary human cell derived
Physiologically relevant phenotype analogous to in-vivo differentiated renal cells
Homogenous, stable and long lasting

Concentration
Vial has between 1-5 million cells as standard, however this may vary.
Cellosaurus ID CVCL_W186

References

There are 8 reference entries for this reagent.

View All References

References: 8 entries

Qin et al. 2020. Med Sci Monit. 26:e919415. PMID: 32053576.

Fan et al. 2019. J Cell Physiol. 234(10):17505-17513. PMID: 30815865.

Ignarski et al. 2019. Int J Mol Sci. 20(13):. PMID: 31277300.

Da Sacco et al. 2013. PLoS One. 8(12):e81812. PMID: 24349133.

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Podocyte culture: tricks of the trade.

Europe PMC ID: 22591222

Saleem et al. 2002. J Am Soc Nephrol. 13(3):630-8. PMID: 11856766.

A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression.

Europe PMC ID: 11856766


Add a reference

References: 8 entries

Qin et al. 2020. Med Sci Monit. 26:e919415. PMID: 32053576.

Fan et al. 2019. J Cell Physiol. 234(10):17505-17513. PMID: 30815865.

Ignarski et al. 2019. Int J Mol Sci. 20(13):. PMID: 31277300.

Da Sacco et al. 2013. PLoS One. 8(12):e81812. PMID: 24349133.

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Podocyte culture: tricks of the trade.

Saleem et al. 2002. J Am Soc Nephrol. 13(3):630-8. PMID: 11856766.

A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression.


Add a reference