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Human Podocyte Cell Line (CIHP-1)

Invented by Prof Moin Saleem at University Of Bristol

Info

Host Human
Tissue Kidney
Model Primary line
Relevance The Human Podocyte Cell Line (CIHP-1) is a primary cell line isolated from human kidney. Podocytes are highly specialised, terminally differentiated cells with a complex cellular structure. They are a critical component of glomerular filtration. Cell cycle control, growth arrest and differentiation are key to the in-vivo biology of podocytes. The conditionally immortalised podocyte cell line allows an in-vitro process of maturation analogous to the development and maturation of podocytes in-vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte proteins nephrin, podocin, CD2AP and synaptopodin. This robust cell line is extensively characterised and validated, used worldwide with over 100 peer reviewed publications to date.
Conditional Description Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT
Research Area Other
Recommended Growing Conditions RPMI-1640, Insulin-Transferrin-Selenium, Foetal Bovine Serum (10% v/v), Pen/Strep medium at at 33°C or 37°C, 5% CO2.
Notes Applications:
Toxicology studies
Metabolism
Therapeutic targeting
Renal physiology R&D
Co-culture studies (e.g 3D culture model)

Key benefits:
Primary human cell derived
Physiologically relevant phenotype analogous to in-vivo differentiated renal cells
Homogenous, stable and long lasting
Cellosaurus ID CVCL_W186

References: 2 entries

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Saleem et al. 2002. J Am Soc Nephrol. 13(3):630-8. PMID: 11856766.


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References: 2 entries

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Saleem et al. 2002. J Am Soc Nephrol. 13(3):630-8. PMID: 11856766.


Add a reference

References: 2 entries

Ni et al. 2012. Nephrology (Carlton). 17(6):525-31. PMID: 22591222.

Saleem et al. 2002. J Am Soc Nephrol. 13(3):630-8. PMID: 11856766.


Add a reference


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