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MEF ULK1/2 WT (SIM) Cell Line

Invented by Dr Sharon Tooze at The Francis Crick Institute

Info

Catalogue Number 151714
Antigen/Gene or Protein Targets ULK1
Host Mouse
Tissue Embryo
Disease Keywords Cancer, neurodegeneration
Model Knock-Out
Relevance The MEF ULK1/2 WT (SIM) cell line can be used as a wild-type control with ULK1 and ULK2 KO MEF cell lines and is useful for studying ULK1-and ULK-2 dependent processes, including autophagy. Mouse embryonic fibroblasts derived from a wild-type littermate to the ULK1 KO and immortalized by serial passaging (spontaneous transformation).
Production Details Cells were derived from mouse embryos at E13.5. Embryos were decapitated and eviscerated and the remaining tissues were dissociated to obtain MEFs. This cell line is a primary cell line immortalized by SIM.
Research Area Apoptosis and Programmed Cell Death, Cell Signaling & Signal Transduction, Metabolism, Neurobiology
Growth/Phenotype Keywords Autophagy
Recommended Growing Conditions DMEM + 20% FCS + 2mM Glutamine + pen/strep
Cellosaurus ID CVCL_5A50

References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.


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References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.


Add a reference

References: 2 entries

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.


Add a reference


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