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Recombinant Antibodies

Ab289 Anti-JUNV GP1 recombinant antibody

Invented by Ron Diskin
Invented at Weizmann Institute Of Science
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GP1JUNV as opposed to GP1LASV elicits a neutralizing response in mice. (A) The infectivity of pseudotyped viruses bearing the spike complexes of JUNV and LASV was tested in the presence of serum samples at 1:250 dilutions from the various GP1JUNV- and GP1LASV-immunized mice. Sera from unimmunized mice were used as controls, and the data were normalized to the values for those sera. Bars represent average values from three independent experiments, each consisting of several technical replicates. Error bars represent standard deviations. Statistically significant reductions in infectivity were determined by a two-tailed Student's t test. *, P < 0.05; **, P < 0.005; ***, P < 0.0005. (B) Dose-response neutralization by GP1JUNV-immunized mouse sera. Graphs show representative results from dose-response experiments using a serum dilution range of 1:250 to 1:8,000 for each of the four mice immunized with GP1JUNV. Mean values from technical replicates are shown, and error bars show standard deviations. Each experiment was repeated at least twice. (C) Complement does not enhance serum neutralization potentials. (Left) Enhanced neutralization of pseudotyped viruses by anti-JUNV Ab289 at 1 μg/ml in the presence of 2.5% complement (red bar) compared to neutralization without the addition of complement (blue bar) as a positive control. The difference in neutralization was significant (two-tailed Student's t test, P < 0.005). Sera from GP1JUNV-immunized mice (middle) and from GP1LASV-immunized mice (right) were tested for neutralization of the corresponding pseudotyped viruses at 1:1,000 and 1:500 dilutions of sera (for GP1JUNV- and GP1LASV-immunized mice, respectively) in the presence or absence of 2.5% complement. None of the differences in neutralization was statistically significant. Results are the averages from three independent experiments, each one of which included several technical replicates.
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Catalogue Number 160881
Applications ELISA FACS IHC IP
Antigen/Gene or Protein Targets GP1 from JUNV
Reactivity Human
Relevance a potent neutralizing Ab against Junín virus (JUNV)
Host Mouse
Immunogen portions of GP1 from JUNV (amino acids 77 to 246 of GPC) (see publication for details).
Subclass IgG1
Molecular Weight (kDa) ~150kDa
Research Area Immunology, Virology
Immunogen UniProt ID UniProtKB - P26313 (GLYC_JUNIN)

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References: 1 entry

Borenstein-Katz et al. 2019. J Virol. 93(8):. PMID: 30728269.

ELISA IHC IP

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References: 1 entry

Borenstein-Katz et al. 2019. J Virol. 93(8):. PMID: 30728269.

ELISA IHC IP

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Inventor Information