HtrA3 Monoclonal Antibody (Clone 3E6)

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References (3)

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Catalogue Number	154229
Applications	IHC WB
Antigen/Gene or Protein Targets	Mouse HtrA3 (high temperature requirement factor A)
Synonyms	HtrA3
Reactivity	Mouse
Relevance	Potential marker for preeclampsia. Detection of endogenous HtrA3 proteins in mouse serum. HtrA3 are oligomeric serine proteases involved in protein quality control , regulate signal transduction in cell growth, apoptosis, inflammation in cancer, arthritis, neurodegenerative and neuromuscuclar disorders, age-related macular degeneration, pregnancy-specific disease preecampsia
Host	Mouse
Immunogen	HtrA3-L-S305A protein
Immunogen UniProt ID	P83110, HTRA3_HUMAN
Molecular Weight (kDa)	48 kDa (46.9 kDa plus 1 kDa for the His tag)
Myeloma Used	Sp2/0-Ag14
Recommended Growing Conditions	Sp2/0 myeloma cells in the presence of 50% (w/v) polyethylene glycol 1500. Cells resuspendedin Hybridoma Serum Free Medium (Invitrogen), supplemented with 10% (w/v) FCS, HAT medium (Invitrogen) and IL-6 (1:1000 dilution).
Strain	Balb/c
Notes	This clone ican be used in WB and IHC for mouse studies (mice only have one isoform of HtrA3), potential diagnostic biomarker for early detection of preecampsia, used at 50 ug/ml final concentration for WB, 8 ug/ml for IHC
Research Area	Gynaecaology, Reproductive Biology

There are 3 reference entries for this reagent.

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27932697

HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening.

Europe PMC ID: 23049902

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Dynon et al. 2012. PLoS One. 7(9):e45956. PMID: 23049902.

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27932697

HtrA3 as an early marker for preeclampsia: specific monoclonal antibodies and sensitive high-throughput assays for serum screening.

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Dynon et al. 2012. PLoS One. 7(9):e45956. PMID: 23049902.

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	Hudson Institute Of Medical Research
	16 reagents listed on Ximbio

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