Anti-HLA-A2 [BB7.2]
Invented by Prof Walter Bodmer at University of Oxford
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![Image thumbnail for Anti-HLA-A2 [BB7.2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,h_45/e1599227-caf5-480f-bed6-52ae34f1c2a2.jpg)
| Catalogue Number | 151297 |
| Applications | FACS IHC IF Fn |
| Antigen/Gene or Protein Targets | Human Leukocyte Antigen- A2 |
| Reactivity | Human |
| Relevance | Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing. |
| Host | Mouse |
| Immunogen | Papain solubilised HLA-A2, |
| Subclass | IgG2b |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
BB7.2 fixes complement and is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. Chawla et al., 2016 - BB7.2 blocked the HLAA2/T cell receptor interactions and specifically killied target cells by E75-CTL. |
| Research Area | Immunology |
References: 42 entries
Huang et al. 2016 Scientific Reports.
FACS
Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.
Europe PMC ID: 27278097
Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.
Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.
Europe PMC ID: 27062199
Reches et al. 2016. J Immunol. :. PMID: 27194785.
FACS
HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
Europe PMC ID: 27194785
Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.
Fn
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Europe PMC ID: 27129972
Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.
Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
Europe PMC ID: 26842468
Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.
FACS
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Europe PMC ID: 26999600
Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.
Europe PMC ID: 26974162
Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.
Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.
Europe PMC ID: 26854383
Srivastava et al. 2016. J Virol. :. PMID: 26842468.
FACS
Ma et al. 2016. J Immunol. :. PMID: 26792804.
FACS
Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.
Europe PMC ID: 26792804
Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.
FACS
Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.
Europe PMC ID: 26787826
Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.
IHC IF
Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.
Europe PMC ID: 26398429
Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.
Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.
Europe PMC ID: 26258424
Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P
Europe PMC ID: 25878105
Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.
FACS
Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.
Europe PMC ID: 26067064
Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.
Khan et al. 2015. J Virol. :. PMID: 25878105.
Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.
Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.
Europe PMC ID: 25852061
Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.
Europe PMC ID: 25816096
Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Europe PMC ID: 24082146
Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.
HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.
Europe PMC ID: 21521873
Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.
Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Europe PMC ID: 7035415
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Europe PMC ID: 95015
Add a reference
References: 42 entries
Huang et al. 2016 Scientific Reports.
FACS
Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.
Europe PMC ID: 27278097
Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.
Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.
Europe PMC ID: 27062199
Reches et al. 2016. J Immunol. :. PMID: 27194785.
FACS
HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
Europe PMC ID: 27194785
Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.
Fn
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Europe PMC ID: 27129972
Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.
Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
Europe PMC ID: 26842468
Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.
FACS
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Europe PMC ID: 26999600
Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.
Europe PMC ID: 26974162
Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.
Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.
Europe PMC ID: 26854383
Srivastava et al. 2016. J Virol. :. PMID: 26842468.
FACS
Ma et al. 2016. J Immunol. :. PMID: 26792804.
FACS
Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.
Europe PMC ID: 26792804
Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.
FACS
Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.
Europe PMC ID: 26787826
Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.
IHC IF
Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.
Europe PMC ID: 26398429
Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.
Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.
Europe PMC ID: 26258424
Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P
Europe PMC ID: 25878105
Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.
FACS
Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.
Europe PMC ID: 26067064
Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.
Khan et al. 2015. J Virol. :. PMID: 25878105.
Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.
Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.
Europe PMC ID: 25852061
Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.
Europe PMC ID: 25816096
Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Europe PMC ID: 24082146
Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.
HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.
Europe PMC ID: 21521873
Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.
Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Europe PMC ID: 7035415
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Europe PMC ID: 95015
Add a reference
References: 42 entries
Huang et al. 2016 Scientific Reports.
FACS
Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.
Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.
Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.
Reches et al. 2016. J Immunol. :. PMID: 27194785.
FACS
HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.
Fn
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.
Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.
FACS
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.
Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.
Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.
Srivastava et al. 2016. J Virol. :. PMID: 26842468.
FACS
Ma et al. 2016. J Immunol. :. PMID: 26792804.
FACS
Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.
Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.
FACS
Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.
Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.
IHC IF
Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.
Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.
Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.
Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P
Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.
FACS
Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.
Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.
Khan et al. 2015. J Virol. :. PMID: 25878105.
Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.
Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.
Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.
Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.
HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.
Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.
Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Add a reference
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