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Anti-HLA-A2 [BB7.2]

Invented by Prof Walter Bodmer at University of Oxford

Info

Catalogue Number 151297
Applications FACS IHC IF Fn
Antigen/Gene or Protein Targets Human Leukocyte Antigen- A2
Reactivity Human
Relevance Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.
Host Mouse
Immunogen Papain solubilised HLA-A2,
Subclass IgG2b
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes BB7.2 fixes complement and is cytotoxic for peripheral blood leukocytes and lymphoid cell lines.

Chawla et al., 2016 - BB7.2 blocked the HLAA2/T cell receptor interactions and specifically killied target cells by E75-CTL.
Research Area Immunology

References: 42 entries

Huang et al. 2016 Scientific Reports.

FACS

Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.

Europe PMC ID: 27278097

Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.

Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.

Europe PMC ID: 27062199

Reches et al. 2016. J Immunol. :. PMID: 27194785.

FACS

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Europe PMC ID: 27194785

Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.

Fn

Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.

Europe PMC ID: 27129972

Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.

Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.

Europe PMC ID: 26842468

Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.

FACS

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Europe PMC ID: 26999600

Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.

Europe PMC ID: 26974162

Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.

Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.

Europe PMC ID: 26854383

Srivastava et al. 2016. J Virol. :. PMID: 26842468.

FACS

Ma et al. 2016. J Immunol. :. PMID: 26792804.

FACS

Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.

Europe PMC ID: 26792804

Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.

FACS

Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.

Europe PMC ID: 26787826

Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.

IHC IF

Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.

Europe PMC ID: 26398429

Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.

Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.

Europe PMC ID: 26258424

Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P

Europe PMC ID: 25878105

Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.

FACS

Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.

Europe PMC ID: 26067064

Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.

Khan et al. 2015. J Virol. :. PMID: 25878105.

Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.

Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.

Europe PMC ID: 25852061

Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.

Europe PMC ID: 25816096

Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.

Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Europe PMC ID: 24082146

Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.

HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.

Europe PMC ID: 21521873

Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.

Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.

Europe PMC ID: 7035415

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.

Europe PMC ID: 95015


Add a reference

References: 42 entries

Huang et al. 2016 Scientific Reports.

FACS

Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.

Europe PMC ID: 27278097

Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.

Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.

Europe PMC ID: 27062199

Reches et al. 2016. J Immunol. :. PMID: 27194785.

FACS

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Europe PMC ID: 27194785

Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.

Fn

Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.

Europe PMC ID: 27129972

Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.

Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.

Europe PMC ID: 26842468

Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.

FACS

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Europe PMC ID: 26999600

Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.

Europe PMC ID: 26974162

Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.

Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.

Europe PMC ID: 26854383

Srivastava et al. 2016. J Virol. :. PMID: 26842468.

FACS

Ma et al. 2016. J Immunol. :. PMID: 26792804.

FACS

Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.

Europe PMC ID: 26792804

Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.

FACS

Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.

Europe PMC ID: 26787826

Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.

IHC IF

Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.

Europe PMC ID: 26398429

Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.

Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.

Europe PMC ID: 26258424

Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P

Europe PMC ID: 25878105

Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.

FACS

Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.

Europe PMC ID: 26067064

Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.

Khan et al. 2015. J Virol. :. PMID: 25878105.

Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.

Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.

Europe PMC ID: 25852061

Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.

Europe PMC ID: 25816096

Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.

Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Europe PMC ID: 24082146

Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.

HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.

Europe PMC ID: 21521873

Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.

Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.

Europe PMC ID: 7035415

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.

Europe PMC ID: 95015


Add a reference

References: 42 entries

Huang et al. 2016 Scientific Reports.

FACS

Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.

Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.

Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.

Reches et al. 2016. J Immunol. :. PMID: 27194785.

FACS

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.

Fn

Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.

Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.

Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.

Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.

FACS

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.

Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.

Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.

Srivastava et al. 2016. J Virol. :. PMID: 26842468.

FACS

Ma et al. 2016. J Immunol. :. PMID: 26792804.

FACS

Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.

Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.

FACS

Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.

Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.

IHC IF

Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.

Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.

Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.

Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P

Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.

FACS

Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.

Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.

Khan et al. 2015. J Virol. :. PMID: 25878105.

Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.

Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.

Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.

Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.

Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.

HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.

Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.

Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.


Add a reference


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