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Anti-HLA-A2 [BB7.2]
RECENTLY UPDATED

Invented by Prof Walter Bodmer at University of Oxford

Info

Catalogue Number 151297
Applications
RECENTLY UPDATED
FACS IHC IF Fn
Antigen/Gene or Protein Targets Human Leukocyte Antigen- A2
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody directed against HLA-A2 histocompatibility antigen, used to aid investigation into foreign antigen presentation and HLA mRNA production.

Background and Research Application
Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). The polymorphic HLA-A, -B, -C glycoproteins have a molecular weight of 43,000 daltons and are expressed on the surfaces of nearly all somatic cells, except red blood cells.
This antibody can be used for HLA typing. Anti-HLA-A2 BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by the A.02 allele. It recognises an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.
Anti-HLA-A2 BB7.2 binds to an epitope at the C-terminus of the alpha-2 helix within the human HLA-A2 histocompatibility antigen. This antibody has exhibited blocking activity. Anti-HLA monoclonal antibodies can help identify in vitro translation production from HLA region mRNA, alongside isolating specific mRNA via precipitation or binding of polysomes. Anti-HLA-A2 can aid study within the immune response, including the presentation of foreign antigens to the immune system.
Host Mouse
Immunogen Papain solubilised HLA-A2,
Immunogen UniProt ID
RECENTLY UPDATED
P04439
Subclass IgG2b
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-HLA-A2 was reported to have recognised HLA-A28. This antibody tested positive for complement dependant cytotoxicity and protein A binding.

BB7.2 fixes complement and is cytotoxic for peripheral blood leukocytes and lymphoid cell lines.

Chawla et al., 2016 - BB7.2 blocked the HLAA2/T cell receptor interactions and specifically killied target cells by E75-CTL.

Concentration
1mg/ml as standard
Research Area
RECENTLY UPDATED
Immunology

References

There are 47 reference entries for this reagent.

View All References

References: 47 entries
RECENTLY UPDATED

Dawson et al. 2019. JCI Insight. 4(6):. PMID: 30753169.

Durgeau et al. 2018. Nat Commun. 9(1):5097. PMID: 30504837.

Dirk et al. 2016. Sci Rep. 6:37021. PMID: 27841315.

Huang et al. 2016 Scientific Reports.

FACS

Nayak et al. 2016. Am J Transplant. 16(8):2300-11. PMID: 27062199.

Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.

Europe PMC ID: 27278097

Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.

Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.

Europe PMC ID: 27062199

Reches et al. 2016. J Immunol. :. PMID: 27194785.

FACS

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Europe PMC ID: 27194785

Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.

Fn

Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.

Europe PMC ID: 27129972

Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.

Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.

Europe PMC ID: 26842468

Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.

FACS

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Europe PMC ID: 26999600

Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.

Europe PMC ID: 26974162

Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.

Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.

Europe PMC ID: 26854383

Srivastava et al. 2016. J Virol. :. PMID: 26842468.

FACS

Ma et al. 2016. J Immunol. :. PMID: 26792804.

FACS

Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.

Europe PMC ID: 26792804

Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.

FACS

Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.

Europe PMC ID: 26787826

Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.

IHC IF

Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.

Europe PMC ID: 26398429

Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.

Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.

Europe PMC ID: 26258424

Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P

Europe PMC ID: 25878105

Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.

FACS

Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.

Europe PMC ID: 26067064

Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.

Khan et al. 2015. J Virol. :. PMID: 25878105.

Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.

Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.

Europe PMC ID: 25852061

Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.

Europe PMC ID: 25816096

Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.

Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Europe PMC ID: 24082146

Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.

HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.

Europe PMC ID: 21521873

Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.

Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.

Europe PMC ID: 7035415

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.

Europe PMC ID: 95015


Add a reference

References: 47 entries
RECENTLY UPDATED

Dawson et al. 2019. JCI Insight. 4(6):. PMID: 30753169.

Durgeau et al. 2018. Nat Commun. 9(1):5097. PMID: 30504837.

Dirk et al. 2016. Sci Rep. 6:37021. PMID: 27841315.

Huang et al. 2016 Scientific Reports.

FACS

Nayak et al. 2016. Am J Transplant. 16(8):2300-11. PMID: 27062199.

Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.

Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.

Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.

Reches et al. 2016. J Immunol. :. PMID: 27194785.

FACS

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.

Fn

Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.

Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.

Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.

The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.

Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.

FACS

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.

Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.

Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.

Srivastava et al. 2016. J Virol. :. PMID: 26842468.

FACS

Ma et al. 2016. J Immunol. :. PMID: 26792804.

FACS

Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.

Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.

FACS

Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.

Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.

IHC IF

Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.

Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.

Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.

Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P

Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.

FACS

Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.

Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.

Khan et al. 2015. J Virol. :. PMID: 25878105.

Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.

Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.

Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.

Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.

Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.

HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.

Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.

Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.


Add a reference