Anti-HLA-A2 [BB7.2]
Invented by Prof Walter Bodmer from University of Oxford
Invented at University of Oxford
- Datasheet
- References (47)
- Inventor Info
Info
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| Catalogue Number | 151297 |
| Applications | FACS IHC IF Fn |
| Antigen/Gene or Protein Targets | Human Leukocyte Antigen- A2 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against HLA-A2 histocompatibility antigen, used to aid investigation into foreign antigen presentation and HLA mRNA production. Background and Research Application Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). The polymorphic HLA-A, -B, -C glycoproteins have a molecular weight of 43,000 daltons and are expressed on the surfaces of nearly all somatic cells, except red blood cells. This antibody can be used for HLA typing. Anti-HLA-A2 BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by the A.02 allele. It recognises an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands. Anti-HLA-A2 BB7.2 binds to an epitope at the C-terminus of the alpha-2 helix within the human HLA-A2 histocompatibility antigen. This antibody has exhibited blocking activity. Anti-HLA monoclonal antibodies can help identify in vitro translation production from HLA region mRNA, alongside isolating specific mRNA via precipitation or binding of polysomes. Anti-HLA-A2 can aid study within the immune response, including the presentation of foreign antigens to the immune system. |
| Host | Mouse |
| Immunogen | Papain solubilised HLA-A2, |
| Immunogen UniProt ID | P04439 |
| Subclass | IgG2b |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-HLA-A2 was reported to have recognised HLA-A28. This antibody tested positive for complement dependant cytotoxicity and protein A binding. BB7.2 fixes complement and is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. Chawla et al., 2016 - BB7.2 blocked the HLAA2/T cell receptor interactions and specifically killied target cells by E75-CTL. Concentration 1mg/ml as standard |
| Research Area | Immunology |
References: 47 entries
Dawson et al. 2019. JCI Insight. 4(6):. PMID: 30753169.
Durgeau et al. 2018. Nat Commun. 9(1):5097. PMID: 30504837.
Dirk et al. 2016. Sci Rep. 6:37021. PMID: 27841315.
Huang et al. 2016 Scientific Reports.
FACS
Nayak et al. 2016. Am J Transplant. 16(8):2300-11. PMID: 27062199.
Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.
Europe PMC ID: 27278097
Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.
Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.
Europe PMC ID: 27062199
Reches et al. 2016. J Immunol. :. PMID: 27194785.
FACS
HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
Europe PMC ID: 27194785
Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.
Fn
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Europe PMC ID: 27129972
Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.
Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
Europe PMC ID: 26842468
Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.
FACS
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Europe PMC ID: 26999600
Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.
Europe PMC ID: 26974162
Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.
Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.
Europe PMC ID: 26854383
Srivastava et al. 2016. J Virol. :. PMID: 26842468.
FACS
Ma et al. 2016. J Immunol. :. PMID: 26792804.
FACS
Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.
Europe PMC ID: 26792804
Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.
FACS
Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.
Europe PMC ID: 26787826
Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.
IHC IF
Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.
Europe PMC ID: 26398429
Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.
Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.
Europe PMC ID: 26258424
Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P
Europe PMC ID: 25878105
Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.
FACS
Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.
Europe PMC ID: 26067064
Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.
Khan et al. 2015. J Virol. :. PMID: 25878105.
Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.
Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.
Europe PMC ID: 25852061
Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.
Europe PMC ID: 25816096
Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Europe PMC ID: 24082146
Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.
HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.
Europe PMC ID: 21521873
Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.
Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Europe PMC ID: 7035415
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Europe PMC ID: 95015
Add a reference
References: 47 entries
Dawson et al. 2019. JCI Insight. 4(6):. PMID: 30753169.
Durgeau et al. 2018. Nat Commun. 9(1):5097. PMID: 30504837.
Dirk et al. 2016. Sci Rep. 6:37021. PMID: 27841315.
Huang et al. 2016 Scientific Reports.
FACS
Nayak et al. 2016. Am J Transplant. 16(8):2300-11. PMID: 27062199.
Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection.
Gunasekaran et al. 2016. Am J Transplant. :. PMID: 27278097.
Long-Term Persistence of Donor Alveolar Macrophages in Human Lung Transplant Recipients That Influences Donor-Specific Immune Responses.
Reches et al. 2016. J Immunol. :. PMID: 27194785.
FACS
HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
Chawla et al. 2016. Cancer Immunol Immunother. :. PMID: 27129972.
Fn
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Srivastava et al. 2016. J Virol. 90(8):3913-28. PMID: 26842468.
Nayak et al. 2016. Am J Transplant. :. PMID: 27062199.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
MacDonald et al. 2016. J Clin Invest. 126(4):1413-24. PMID: 26999600.
The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
Lasso et al. 2016. PLoS One. 11(3):e0150996. PMID: 26974162.
FACS
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.
Lasso et al. 2016. Peptides. 78:68-76. PMID: 26854383.
Effect of secondary anchor amino acid substitutions on the immunogenic properties of an HLA-A*0201-restricted T cell epitope derived from the Trypanosoma cruzi KMP-11 protein.
Srivastava et al. 2016. J Virol. :. PMID: 26842468.
FACS
Ma et al. 2016. J Immunol. :. PMID: 26792804.
FACS
Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules.
Mahiti et al. 2016. MBio. 7(1):. PMID: 26787826.
FACS
Relative Resistance of HLA-B to Downregulation by Naturally Occurring HIV-1 Nef Sequences.
Horiuchi et al. 2015. Oncol Rep. :. PMID: 26398429.
IHC IF
Targeting cryptic epitope with modified antigen coupled to the surface of liposomes induces strong antitumor CD8 T-cell immune responses in vivo.
Dellgren et al. 2015. PLoS One. 10(8):e0135385. PMID: 26258424.
Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains.
Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local P
Rancan et al. 2015. PLoS Pathog. 11(6):e1004906. PMID: 26067064.
FACS
Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells.
Egui et al. 2015. PLoS One. 10(3):e0122115. PMID: 25816096.
Khan et al. 2015. J Virol. :. PMID: 25878105.
Noy et al. 2015. Mol Cancer Ther. :. PMID: 25852061.
Recruitment of Oligoclonal Viral-Specific T cells to Kill Human Tumor Cells Using Single-Chain Antibody-Peptide-HLA Fusion Molecules.
Differential phenotypic and functional profiles of TcCA-2 -specific cytotoxic CD8+ T cells in the asymptomatic versus cardiac phase in Chagasic patients.
Cheent et al. 2013. Proc Natl Acad Sci U S A. 110(42):16981-6. PMID: 24082146.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Whitfield-Larry et al. 2011. Diabetes. 60(6):1726-33. PMID: 21521873.
HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.
Parham et al. 1981. Hum Immunol. 3(4):277-99. PMID: 7035415.
Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Add a reference
