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Monoclonal Antibodies

Anti-PHD2 [366G/76/3]

Invented by Helen Turley from University of Oxford
Invented at University of Oxford
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Adapted from Appelhoff et al. 2004. J Biol Chem; 279(37):38458-65. PMID: 15247232 Figure. Effect of estrogen on PHD expression. BT-474 cells were incubated with (+) or without (–) 20 ng/ml estradiol for 48 h. Whole cell extracts were immunoblotted with antibodies against PHD1, PHD2, and PHD3.
Adapted from Stolze et al. 2004. J Biol Chem; 279(41):42719-25. PMID: 15302861 Figure. Immunoblot analysis for HIF-1α, PHD1–3, and FIH using whole cell extracts from HeLa and U-2OS cells treated with control (–) or FIH (+) siRNA prior parallel incubation in normoxia (N) and hypoxia (H, 16 h, 1% oxygen).
Adapted from Soilleux et al. 2005. Histopathology; 47(6):602-10. PMID: 16324198 Figure. Immunostaining of transfected cell lines and human tissues for prolyl hydroxylase PHD‐2. Immunostaining of PHD‐2 (A2)-transfected COS cells. Immunostaining of normal kidney with PHD‐2 (B2). Immunostaining of a renal cell carcinoma with PHD‐2 (C2). Immunostaining of a follicular lymphoma with PHD‐2 (D2).
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Catalogue Number 151314
Applications FACS IHC WB
Antigen/Gene or Protein Targets Prolyl Hydroxylase 2 (PHD2)
Reactivity Human
Relevance 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
Host Mouse
Immunogen Residues 1-24 of PHD2
Positive Control MCF7 cells
Subclass IgG1
Molecular Weight (kDa) 46.1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS + 5% CO2
Strain Balb/c
Research Area Cancer, Cardiovascular, Epigenetics & Nuclear Signalling, Metabolism

References

There are 8 reference entries for this reagent.

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References: 8 entries

Jubb et al. 2009. Br J Cancer. 101(10):1749-57. PMID: 19844231.

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Expression of delta-like ligand 4 (Dll4) and markers of hypoxia in colon cancer.

Europe PMC ID: 19844231

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Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.

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Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.

Europe PMC ID: 16324198

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Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.

View  

Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.

View  

Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].

Europe PMC ID: 15302861

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Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.

Europe PMC ID: 15247232

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References: 8 entries

Jubb et al. 2009. Br J Cancer. 101(10):1749-57. PMID: 19844231.

View  

Expression of delta-like ligand 4 (Dll4) and markers of hypoxia in colon cancer.

View  

Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.

View  

Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.

View  

Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.

View  

Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.

View  

Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].

View  

Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.

View  

Add a reference

Inventor Information