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Catalogue Number 152726
Backbone Size (bp) 5169
Bacterial Resistance Puromycin
Vector Type pBabe-puro3
Antigen/Gene or Protein Targets Rock2, RockII
Relevance Rock2 (Cow):ER GFP Plasmid contains kinase domain along with part of the coiled–coil region of bovine Rock2 (residues 5–553) attached to the hormone‐binding domain of the mouse estrogen receptor (hbER) at the carboxyl‐terminus and enhanced green fluorescent protein (EGFP) at the amino terminus.

Rock2 (Cow):ER GFP Plasmid facilitates the study of Rock signaling in a more regulated manner by offering a conditionally active form of Rock2 through its fusion to the hormone‐binding domain of the estrogen receptor (ER). Activation can be achieved using the estrogen antagonist 4‐hydroxytamoxifen (4‐HT). Ultimately, Rock2 activation leads to phosphorylation of proteins involved in the regulation of the actin‐myosin cytoskeleton, generation of pronounced actin stress fibers, and changes in cell morphology. Rock2 (Cow):ER GFP serves as a good complement for small‐molecule Rock2 inhibitors.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility

References: 1 entry

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.


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References: 1 entry

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.


Add a reference

References: 1 entry

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.


Add a reference


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