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Rock2 (Cow):ER GFP Vector

Invented by Prof Michael Olson at Cancer Research UK Glasgow: The Beatson Institute
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Schematic representation of Rock2 and the conditionally regulated Rock (Cow):ER showing the functional domains; PH, Pleckstrin homology domain, CRT, cysteine-rich domain. Amino acids 5-553 were joined in frame to eGFP and estrogen receptor hormone binding domain (hbER) moieties to create the Rock (Cow):ER fusion protein. A kinase dead version (Rock2 (Cow):ER-KD) was created by changing lysine 121 to glycine (K121G).
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Catalogue Number 152726
Backbone Size (bp) 5169
Bacterial Resistance Puromycin
Vector Type pBabe-puro3
Antigen/Gene or Protein Targets Rock2, RockII
Relevance Rock2 (Cow):ER GFP Plasmid contains kinase domain along with part of the coiled–coil region of bovine Rock2 (residues 5–553) attached to the hormone‐binding domain of the mouse estrogen receptor (hbER) at the carboxyl‐terminus and enhanced green fluorescent protein (EGFP) at the amino terminus.

Rock2 (Cow):ER GFP Plasmid facilitates the study of Rock signaling in a more regulated manner by offering a conditionally active form of Rock2 through its fusion to the hormone‐binding domain of the estrogen receptor (ER). Activation can be achieved using the estrogen antagonist 4‐hydroxytamoxifen (4‐HT). Ultimately, Rock2 activation leads to phosphorylation of proteins involved in the regulation of the actin‐myosin cytoskeleton, generation of pronounced actin stress fibers, and changes in cell morphology. Rock2 (Cow):ER GFP serves as a good complement for small‐molecule Rock2 inhibitors.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility

References: 2 entries

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.

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Conditional regulation of a ROCK-estrogen receptor fusion protein.

Europe PMC ID: 16472686

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References: 2 entries

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.

View  

Conditional regulation of a ROCK-estrogen receptor fusion protein.

Europe PMC ID: 16472686

View  

Add a reference

References: 2 entries

Croft et al. 2006. Methods Enzymol. 406:541-53. PMID: 16472686.

View  

Conditional regulation of a ROCK-estrogen receptor fusion protein.

View  

Add a reference

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