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Anti-Nucleophosmin [NA24]

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151399
Applications ChIP IHC IF IP WB
Antigen/Gene or Protein Targets Nucleophosmin
Synonyms Nucleolar Phosphoprotein B23; Nucleolar Protein NO38; Numatrin; NPM; Nucleophosmin/Nucleoplasmin Family; Testicular Tissue Protein Li 128; B23
Reactivity Human
Relevance Monoclonal antibody with use in understanding various nucleophosmin mutations and their associated diseases.

Background and Research Application
Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. NPM also localises between the paired centrioles of the centrosome and dissociates upon the phosphorylation of on Thr199 by CDK2/cyclin E prior to the initiation of centrosome duplication, an essential process for successful chromosome segregation during mitosis. The down-regulation of this protein results in the abnormal amplification of centrosomes which suggests that this protein may act as a suppressor of centrosome duplication. Aberrations involving nucleophasmin are found in multiple conditions, for instance in a form of non-Hodgkin lymphoma or acute promyelocytic leukaemia.
The epitope for this antibody lies within the N-terminal of NPM. It reacts with both WT-NPM and NPM-ALK, staining nuclei of neoplastic cells and providing diffuse cytoplasmic labelling.
Host Mouse
Immunogen Nucleophosmin recombinant protein
Immunogen UniProt ID P06748
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Ultroser G can be used at 1% if the cells are not growing well.
NE24 antibody was used to perform immunohistochemical staining confirming that anaplastic large-cell lymphoma involves expression of mutated NPM-ALK fusion.

Concentration
1mg/ml as standard
Research Area Epigenetics & Nuclear Signalling

References

There are 11 reference entries for this reagent.

View All References

References: 11 entries

Vitamin D Receptor Expression in Plasmablastic Lymphoma and Myeloma Cells Confers Susceptibility to Vitamin D.

Europe PMC ID: 28001444

Gascoyne et al. 2016. Endocrinology. :en20161802. PMID: 28001444.

WB

Wong et al. 2016. Oncotarget. 7(33):52940-52956. PMID: 27224915.

Raman et al. 2014. Mol Cell Biol. 34(24):4474-84. PMID: 25288641.

IF WB

mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3.

Europe PMC ID: 25288641

Finkbeiner et al. 2011. EMBO J. 30(6):1067-78. PMID: 21326211.

IF WB

The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.

Europe PMC ID: 21326211

Gimenez et al. 2010. Proteomics. 10(15):2812-21. PMID: 20533335.

Proteomic analysis of low- to high-grade astrocytomas reveals an alteration of the expression level of raf kinase inhibitor protein and nucleophosmin.

Europe PMC ID: 20533335

Cordell et al. 1999. Blood. 93(2):632-42. PMID: 9885226.

IHC WB

Detection of normal and chimeric nucleophosmin in human cells.

Europe PMC ID: 9885226


Add a reference

References: 11 entries

Vitamin D Receptor Expression in Plasmablastic Lymphoma and Myeloma Cells Confers Susceptibility to Vitamin D.

Gascoyne et al. 2016. Endocrinology. :en20161802. PMID: 28001444.

WB

Wong et al. 2016. Oncotarget. 7(33):52940-52956. PMID: 27224915.

Raman et al. 2014. Mol Cell Biol. 34(24):4474-84. PMID: 25288641.

IF WB

mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3.

Finkbeiner et al. 2011. EMBO J. 30(6):1067-78. PMID: 21326211.

IF WB

The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.

Gimenez et al. 2010. Proteomics. 10(15):2812-21. PMID: 20533335.

Proteomic analysis of low- to high-grade astrocytomas reveals an alteration of the expression level of raf kinase inhibitor protein and nucleophosmin.

Cordell et al. 1999. Blood. 93(2):632-42. PMID: 9885226.

IHC WB

Detection of normal and chimeric nucleophosmin in human cells.


Add a reference