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|Applications||ChIP IHC IF IP WB|
|Antigen/Gene or Protein Targets||Nucleophosmin|
|Synonyms||Nucleolar Phosphoprotein B23; Nucleolar Protein NO38; Numatrin; NPM; Nucleophosmin/Nucleoplasmin Family; Testicular Tissue Protein Li 128; B23|
|Relevance||Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. NPM also localises between the paired centrioles of the centrosome and dissociates upon the phosphorylation of on Thr199 by CDK2/cyclin E prior to the initiation of centrosome duplication, an essential process for successul chromosome segregation during mitosis. The down-regulation of this protein results in the abnormal amplification of centrosomes which suggests that this protein may act as a a suppressor of centrosome duplication.|
|Immunogen||Nucleophosmin recombinant protein|
|Recommended Growing Conditions||RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT|
|Notes||Ultroser G can be used at 1% if the cells are not growing well.|
|Research Area||Epigenetics & Nuclear Signalling|
References: 5 entries
Gascoyne et al. 2016. Endocrinology. :en20161802. PMID: 28001444.
Raman et al. 2014. Mol Cell Biol. 34(24):4474-84. PMID: 25288641.
Finkbeiner et al. 2011. EMBO J. 30(6):1067-78. PMID: 21326211.
Gimenez et al. 2010. Proteomics. 10(15):2812-21. PMID: 20533335.
Cordell et al. 1999. Blood. 93(2):632-42. PMID: 9885226.
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