Anti-Nucleophosmin [NA24] mAb

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-Nucleophosmin [NA24] mAb
• Alternate name: Nucleolar Phosphoprotein B23; Nucleolar Protein NO38; Numatrin; NPM; Nucleophosmin/Nucleoplasmin Family; Testicular Tissue Protein Li 128; B23
• Clone: NA24
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: ChIP ; IHC ; IF ; IP ; WB
• Description: Monoclonal antibody with use in understanding various nucleophosmin mutations and their associated diseases.
• Immunogen: Nucleophosmin recombinant protein
• Immunogen uniprot id: P06748
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Nucleophosmin
• Target background: Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. NPM also localises between the paired centrioles of the centrosome and dissociates upon the phosphorylation of on Thr199 by CDK2/cyclin E prior to the initiation of centrosome duplication, an essential process for successful chromosome segregation during mitosis. The down-regulation of this protein results in the abnormal amplification of centrosomes which suggests that this protein may act as a suppressor of centrosome duplication. Aberrations involving nucleophasmin are found in multiple conditions, for instance in a form of non-Hodgkin lymphoma or acute promyelocytic leukaemia. The epitope for this antibody lies within the N-terminal of NPM. It reacts with both WT-NPM and NPM-ALK, staining nuclei of neoplastic cells and providing diffuse cytoplasmic labelling.

Applications

• Application: ChIP ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

Related Tools

• Related tools: Anti-Nucleophosmin [NA24] recombinant antibody

References

• Vitamin D Receptor Expression in Plasmablastic Lymphoma and Myeloma Cells Confers Susceptibility to Vitamin D.
• Gascoyne et al. 2016. Endocrinology. :en20161802. PMID: 28001444.
• Wong et al. 2016. Oncotarget. 7(33):52940-52956. PMID: 27224915.
• Raman et al. 2014. Mol Cell Biol. 34(24):4474-84. PMID: 25288641.
• mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3.
• Finkbeiner et al. 2011. EMBO J. 30(6):1067-78. PMID: 21326211.
• The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.
• Gimenez et al. 2010. Proteomics. 10(15):2812-21. PMID: 20533335.
• Proteomic analysis of low- to high-grade astrocytomas reveals an alteration of the expression level of raf kinase inhibitor protein and nucleophosmin.
• Cordell et al. 1999. Blood. 93(2):632-42. PMID: 9885226.
• Detection of normal and chimeric nucleophosmin in human cells.