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|Applications||IHC IF IP WB|
|Antigen/Gene or Protein Targets||Neutrophil elastase|
Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis.
NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labeling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections
|Immunogen||Neutrophil granule proteins|
|Recommended Growing Conditions||RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT|
|Notes||Ultroser G can be used at 1% if the cells are not growing well.|
|Research Area||Cancer, Cell Signaling & Signal Transduction, Immunology|
References: 8 entries
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Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.
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Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.
Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.
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