Anti-Neutrophil elastase [NP57]
Invented by Karen Pulford from University of Oxford
Invented at University of Oxford
- Datasheet
- References (17)
- Inventor Info
Info
| Catalogue Number | 151398 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Neutrophil elastase |
| Reactivity | Human |
| Relevance |
Monoclonal antibody with use in diagnosis of acute leukaemia and tumour deposits in myeloid leukaemia. Background and Research Application Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis. NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labelling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections. |
| Host | Mouse |
| Immunogen | Neutrophil granule proteins |
| Immunogen UniProt ID | P08246 |
| Subclass | IgG1 kappa |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody recognizes neutrophils and their precursors. The antigen recognised is sharply localized in individual myeloid cells. Ultroser G can be used at 1% if the cells are not growing well. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Immunology |
References: 17 entries
Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus).
Europe PMC ID: 27165445
Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.
IgE and FcεRI are highly expressed on innate cells in psoriasis.
Europe PMC ID: 26853903
Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.
Europe PMC ID: 26887258
Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.
IHC
Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.
Hosseini et al. 2016. Part Fibre Toxicol. 13:2. PMID: 26758251.
Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.
IHC
Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.
Europe PMC ID: 26396242
Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.
IHC
A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.
Europe PMC ID: 26178731
Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.
Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.
Europe PMC ID: 8658372
Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.
Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.
Europe PMC ID: 2759560
Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.
Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.
Europe PMC ID: 2844860
Add a reference
References: 17 entries
Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus).
Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.
IgE and FcεRI are highly expressed on innate cells in psoriasis.
Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.
Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.
IHC
Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.
Hosseini et al. 2016. Part Fibre Toxicol. 13:2. PMID: 26758251.
Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.
IHC
Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.
Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.
IHC
A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.
Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.
Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.
Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.
Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.
Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.
Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.
Add a reference
