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Anti-Neutrophil elastase [NP57]

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151398
Applications IHC IF IP WB
Antigen/Gene or Protein Targets Neutrophil elastase
Reactivity Human
Relevance Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis.



NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labeling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections
Host Mouse
Immunogen Neutrophil granule proteins
Subclass IgG1 kappa
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Signaling & Signal Transduction, Immunology

References: 16 entries

Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus).

Europe PMC ID: 27165445

Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.

IgE and FcεRI are highly expressed on innate cells in psoriasis.

Europe PMC ID: 26853903

Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.

Europe PMC ID: 26887258

Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.

IHC

Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.

Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.

IHC

Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.

Europe PMC ID: 26396242

Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.

IHC

A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.

Europe PMC ID: 26178731

Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.

Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.

Europe PMC ID: 8658372

Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.

Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.

Europe PMC ID: 2759560

Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.

Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.

Europe PMC ID: 2844860


Add a reference

References: 16 entries

Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus).

Europe PMC ID: 27165445

Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.

IgE and FcεRI are highly expressed on innate cells in psoriasis.

Europe PMC ID: 26853903

Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.

Europe PMC ID: 26887258

Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.

IHC

Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.

Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.

IHC

Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.

Europe PMC ID: 26396242

Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.

IHC

A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.

Europe PMC ID: 26178731

Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.

Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.

Europe PMC ID: 8658372

Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.

Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.

Europe PMC ID: 2759560

Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.

Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.

Europe PMC ID: 2844860


Add a reference

References: 16 entries

Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus).

Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.

IgE and FcεRI are highly expressed on innate cells in psoriasis.

Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.

Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.

IHC

Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.

Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.

IHC

Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.

Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.

IHC

A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.

Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.

Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.

Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.

Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.

Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.

Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.


Add a reference


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