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Anti-Neutrophil elastase [NP57]

Info

Catalogue Number 151398
Applications IHC IF IP WB
Antigen/Gene or Protein Targets Neutrophil elastase
Reactivity Human
Relevance Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis.



NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labeling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections
Host Mouse
Immunogen Neutrophil granule proteins
Subclass IgG1 kappa
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Signaling & Signal Transduction, Immunology

References: 8 entries

Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.

Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.

IHC

Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.

Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.

IHC

Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.

IHC

Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.

Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.

Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.


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