Anti-Neutrophil elastase [NP57]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-Neutrophil elastase [NP57]
• Clone: NP57
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 150 kDa (full length)
• Reactivity: Human
• Host: Mouse
• Application: IHC ; IF ; IP ; WB
• Description: Monoclonal antibody with use in diagnosis of acute leukaemia and tumour deposits in myeloid leukaemia.
• Immunogen: Neutrophil granule proteins
• Immunogen uniprot id: P08246
• Isotype: IgG1 kappa
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Neutrophil elastase
• Molecular weight: 150 kDa (full length)
• Target background: Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis. NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labelling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.

Applications

• Application: IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus).
• Bleyer et al. 2016. Exp Toxicol Pathol. :. PMID: 27165445.
• IgE and FceRI are highly expressed on innate cells in psoriasis.
• Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.
• Snäll et al. 2016. Sci Rep. 6:21288. PMID: 26887258.
• Yan et al. 2016. Br J Dermatol. :. PMID: 26853903.
• Hosseini et al. 2016. Part Fibre Toxicol. 13:2. PMID: 26758251.
• Hochman et al. 2015. MBio. 6(5):. PMID: 26396242.
• Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.
• Farrar et al. 2015. Am J Clin Nutr. :. PMID: 26178731.
• A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.
• Nasser et al. 1996. Thorax. 51(1):64-70. PMID: 8658372.
• Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.
• Ralfkiaer et al. 1989. Histopathology. 14(6):637-43. PMID: 2759560.
• Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.
• Pulford et al. 1988. J Clin Pathol. 41(8):853-60. PMID: 2844860.
• Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.