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|Antigen/Gene or Protein Targets||Rabbit GAPDH, TNF-α, MIP-1β, IL-6, IL-8|
Useful for research on gene expression, inflammation and inflammatory disease models.
This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. Multi-gene plasmid reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes.
|Research Area||Gene Expression, Immunology|
Insert size: 700bp
Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
Only one standard curve dilutional series has to be generated instead of 12 individual ones.
Standard curves have been constructed for all 2 chemokines, 7 cytokines and the 2 housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 10^8 copies of target.
Sequence analysis confirms the orientation and authenticity of the cloned gene sequences. All sequences show >98% identity with the DNA sequences deposited in the NCBI database
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