- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||Human IL-2, MIF, Rantes, HPRT, IL-1β, IL-6, IFN-γ, TNF-α, MIP-2α, β-actin, MIP-1α, MIP-1β, MCP-1 and IL-8.|
Useful for research on gene expression, inflammation and inflammatory disease models.
This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. Multi-gene plasmid reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes.
|Research Area||Gene Expression, Immunology|
Insert size: 2400bp
Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
Only one standard curve dilutional series has to be generated instead of 14 individual ones. Standard curves have been constructed for all chemokines, cytokines and the housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 10^8 copies of target.
Sequence analysis confirms the orientation and authenticity of the cloned gene sequences.
All sequences show >98% identity with the DNA sequences deposited in the NCBI database.
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