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- Inventor Info
|Antigen/Gene or Protein Targets||Murine MIG, MIP-ly, MlP-2, MIP-lα, TNF-α, ILI-β IL-6, β-actin MCP2, RANTES, Eotaxin, Fractalkine, Lymphotactin, MIF, IP-I0, MIP-β, and HPRT|
Useful for research on gene expression, inflammation and inflammatory disease models.
This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. Multi-gene plasmid reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes.
|Research Area||Gene Expression, Immunology|
Insert size: 2500bp
Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
Only one standard curve dilutional series has to be generated instead of 17 individual ones. Standard curves have been constructed for all 12 chemokines, 3 cytokines and the 2 housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 10^8 copies of target.
Sequence analysis confirms the orientation and authenticity of the cloned gene sequences.
All sequences show >98% identity with the DNA sequences deposited in the NCBI database.
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