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Catalogue Number
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157885 |
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Antigen/Gene or Protein Targets
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Heteroxylan, specifically it binds strongly to heteroxylan preparations from grass cell walls.
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Synonyms
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Xylan, β-1,4-linked xylose
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Relevance
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Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures.
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Host
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Rat
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Immunogen
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RG-I oligosaccharides coupled to BSA
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Positive Control
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IgM
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Myeloma Used
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IR983F
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Notes
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Two male Wistar rats were injected with 25 μg of RG-I oligosaccharides coupled to BSA in complete Freund’s adjuvant administered sub-cutaneously on day 0, with the same amount administered with incomplete Freund’s adjuvant on days 28 and 63. On day 133, the rat was given a pre-fusion boost of 1 mg immunogen in PBS by intraperitoneal injection. Spleen lymphocytes were isolated 3 days later and fused with rat myeloma cell line IR983F (Bazin 1982). Hybridoma cells were selected by enzyme-linked immunosorbent assay (ELISA) using the coupled oligosaccharides as the immobilised antigen and this led to the isolation of rat MAb LM27.
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Research Area
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Plant Science
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