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Anti-Grass xylan preparations [LM27] monoclonal antibody
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Invented by Prof Paul Knox at University of Leeds

Info

Catalogue Number 157885
Antigen/Gene or Protein Targets Heteroxylan, specifically it binds strongly to heteroxylan preparations from grass cell walls.
Synonyms Xylan, β-1,4-linked xylose
Relevance Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures.
Host Rat
Immunogen RG-I oligosaccharides coupled to BSA
Positive Control IgM
Myeloma Used IR983F
Notes Two male Wistar rats were injected with 25 μg of RG-I oligosaccharides coupled to BSA in complete Freund’s adjuvant administered sub-cutaneously on day 0, with the same amount administered with incomplete Freund’s adjuvant on days 28 and 63. On day 133, the rat was given a pre-fusion boost of 1 mg immunogen in PBS by intraperitoneal injection. Spleen lymphocytes were isolated 3 days later and fused with rat myeloma cell line IR983F (Bazin 1982). Hybridoma cells were selected by enzyme-linked immunosorbent assay (ELISA) using the coupled oligosaccharides as the immobilised antigen and this led to the isolation of rat MAb LM27.
Research Area Plant Science

References

There are 1 reference entries for this reagent.

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References: 1 entry

Cornuault et al. 2015. Planta. 242(6):1321-34. PMID: 26208585.


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References: 1 entry

Cornuault et al. 2015. Planta. 242(6):1321-34. PMID: 26208585.


Add a reference