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Anti-Xenopus NK cells [1G5] monoclonal antibody

Invented by John Horton
Invented at University of Durham

Info

Catalogue Number 157744
Applications FACS IHC WB
Antigen/Gene or Protein Targets Xenopus Natural Killer (NK) cells
Reactivity Xenopus laevis
Relevance Enables identification of specific lymphoid populations in Xenopus (tested in liver, spleen and gut), namely NK cells. Enables separation from T and B cells.
Host Mouse
Immunogen Mice were immunised with splenocytes from early-thymectomized (Tx) Xenopus following B cell and thrombocyte depletion, therefore an enriched Natural Killer (NK) cell population.
Immunogen UniProt ID Not applicable
Positive Control IgG2a kappa
Molecular Weight (kDa) 74
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640 + 10% FCS
Notes Bands at 72 kDa and 74 kDa are found when probing western blots of lysates of Xenopus spleen, liver and gut with antibody 1G5, but an additional band at 84 kDa seen only in spleen and liver lysates indicates a potential difference in expression in these organs. mAb 1F8 and 4D4 behaved relatively poorly in Western blots, but identified protein bands similar to those recognised by 1G5.
Research Area Cancer, Cell Type or Organelle Marker, Immunology

References

There are 1 reference entries for this reagent.

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References: 1 entry

Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.


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References: 1 entry

Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.


Add a reference

Inventor Information