Anti-Xenopus NK cells [1G5] monoclonal antibody

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Catalogue Number	157744
Applications	FACS IHC WB
Antigen/Gene or Protein Targets	Xenopus Natural Killer (NK) cells
Reactivity	Xenopus laevis
Relevance	Enables identification of specific lymphoid populations in Xenopus (tested in liver, spleen and gut), namely NK cells. Enables separation from T and B cells.
Host	Mouse
Immunogen	Mice were immunised with splenocytes from early-thymectomized (Tx) Xenopus following B cell and thrombocyte depletion, therefore an enriched Natural Killer (NK) cell population.
Immunogen UniProt ID	Not applicable
Positive Control	IgG2a kappa
Molecular Weight (kDa)	74
Myeloma Used	P3X63Ag8.653
Recommended Growing Conditions	RPMI 1640 + 10% FCS
Notes	Bands at 72 kDa and 74 kDa are found when probing western blots of lysates of Xenopus spleen, liver and gut with antibody 1G5, but an additional band at 84 kDa seen only in spleen and liver lysates indicates a potential difference in expression in these organs. mAb 1F8 and 4D4 behaved relatively poorly in Western blots, but identified protein bands similar to those recognised by 1G5.
Research Area	Cancer, Cell Type or Organelle Marker, Immunology

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Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.

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Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.

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John Horton

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	University of Durham
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