Anti-Xenopus NK cells [1F8] monoclonal antibody

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Catalogue Number	157742
Applications	FACS IHC
Antigen/Gene or Protein Targets	Xenopus Natural Killer (NK) cells
Reactivity	Xenopus laevis
Relevance	Enables identification of specific lymphoid populations in Xenopus (tested in liver, spleen and gut), namely NK cells. Enables separation from T and B cells.
Host	Mouse
Immunogen	Mice were immunised with splenocytes from early-thymectomized (Tx) Xenopus following B cell and thrombocyte depletion, therefore an enriched Natural Killer (NK) cell population.
Immunogen UniProt ID	Not applicable
Positive Control	IgG2b kappa
Molecular Weight (kDa)	72
Myeloma Used	P3X63Ag8.653
Recommended Growing Conditions	RPMI 1640 + 10% FCS
Notes	Behaves relatively poorly in western blotting, however does identify bands similar to antibody clone 1G5.
Research Area	Cancer, Cell Type or Organelle Marker, Immunology

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Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.

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Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.

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John Horton

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	University of Durham
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