Anti-Xenopus NK cells [1G5] monoclonal antibody
Invented by John Horton at University of Durham
| Catalogue Number | 157743 | 
| Applications | FACS IHC | 
| Antigen/Gene or Protein Targets | Xenopus Natural Killer (NK) cells | 
| Reactivity | Xenopus laevis | 
| Relevance | Enables identification of specific lymphoid populations in Xenopus (tested in liver, spleen and gut), namely NK cells. Enables separation from T and B cells. | 
| Host | Mouse | 
| Immunogen | Mice were immunised with splenocytes from early-thymectomized (Tx) Xenopus following B cell and thrombocyte depletion, therefore an enriched Natural Killer (NK) cell population. | 
| Immunogen UniProt ID | Not applicable | 
| Positive Control | IgG2a kappa | 
| Molecular Weight (kDa) | 72 | 
| Myeloma Used | P3X63Ag8.653 | 
| Recommended Growing Conditions | RPMI 1640 + 10% FCS | 
| Notes | Behaves relatively poorly in western blotting, however does identify bands similar to antibody clone 1G5. | 
| Research Area | Cancer, Cell Type or Organelle Marker, Immunology |