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Anti-Xenopus NK cells [4D4] monoclonal antibody
RECENTLY UPDATED

Invented by John Horton
Invented at University of Durham

Info

Catalogue Number 157743
Applications FACS IHC
Antigen/Gene or Protein Targets Xenopus Natural Killer (NK) cells
Reactivity Xenopus laevis
Relevance Enables identification of specific lymphoid populations in Xenopus (tested in liver, spleen and gut), namely NK cells. Enables separation from T and B cells.
Host Mouse
Immunogen Mice were immunised with splenocytes from early-thymectomized (Tx) Xenopus following B cell and thrombocyte depletion, therefore an enriched Natural Killer (NK) cell population.
Immunogen UniProt ID Not applicable
Positive Control IgG2a kappa
Molecular Weight (kDa) 72
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640 + 10% FCS
Notes Behaves relatively poorly in western blotting, however does identify bands similar to antibody clone 1G5.
Research Area Cancer, Cell Type or Organelle Marker, Immunology

References

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References: 1 entry

Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.


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References: 1 entry

Horton et al. 2000. Eur J Immunol. 30(2):604-13. PMID: 10671217.


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Inventor Information