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hTERT EP156T cell line
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Antigen/Gene or Protein Targets Prostate Cancer
Parental Line EP156T
Host Human
Disease Keywords Prostate Cancer
Model Tumour line
Relevance This cell line presents an in vitro model system which enables the study of human prostate cell differentiation and malignant transformation. Human prostate cells are typically difficult to convert into continuously growing cultures. This cell line is immortalized without viral oncogenes from prostate epithelial cells and may serve as a unique experimental platform to permit several research directions, including the study of cell-cell interactions in an authentic prostate microenvironment, prostate cell differentiation, and most significantly, the complex multistep process leading to prostate cell transformation. PMID:16585177.
Production Details EP156 cells were derived from a radical prostatectomy specimen of a 66-year-old patient. Pathologic tumor stage was pT3a, Gleason score 7 (4 + 3). The specimen had a positive margin, meaning that the tumor extended the prostate capsule. Seminal vesicles were tumor-free. (PMID 16585177).
Conditional No
Research Area Cancer
Growth/Phenotype Keywords Epithelial-like
Recommended Growing Conditions Reccomend culture using MCDB 153 medium supplemented with penicillin/streptomycin antibiotics, 1% of non-essential amino acids solution, 1% of fetal bovine serum, 50 µg/ml of bovine pituitary extract, 5 µg/ml of insulin, 5 µg/ml of transferrin, 5 ng/ml of EGF, 200 nM of hydrocortisone, 10 nM of triiodothyronine, 10 nM of testosterone and 5 ng/ml of sodium selenite. Reccomend growth of cultures in a humidified atmosphere of 5% CO2 in air at 37°C. The medium is reccomended to be changed after 1 week and then every 2 to 3 days. PMID:16585177.
Cellosaurus ID CVCL_RW45

References

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References: 1 entry

Kogan et al. 2006. Cancer Res. 66(7):3531-40. PMID: 16585177.


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References: 1 entry

Kogan et al. 2006. Cancer Res. 66(7):3531-40. PMID: 16585177.


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