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Anti-Human IgG scFv recombinant antibody (HRP)

Info

Catalogue Number 154185
Applications ELISA WB DB
Antigen/Gene or Protein Targets Human IgG
Reactivity Human
Relevance Anti-Human secondary antibody which can be used to detect human primary antibodies
Immunogen Human IgG, whole molecule
Subclass IgG1
Formulation PBS (0.1M) + 0.5M imidazole at pH 7.4
This product was purified using affinity chromatography (Ni-IMAC)
Research Area Secondary Antibody
Notes This product is a recombinant single chain variable fragment (scFv) fused to Horseradish peroxidase (HRP) and produced in N. Benthamiana plants.
Volume: 100ul
Storage: 40C
Form & colour: Reddish/brown liquid
Stability: Up to 1 year from delivery
Conjugation notes: Horseradish Peroxidase isoenzyme C (EC 1.11.1.7) recombinantly fused to N terminus
Clonality: Monoclonal
Host species: Mouse, made in N. Benthamiana plants

Western blot: Suggested working dilution 1/1000-1/5000 to detect 1ug antigen in 5% milk. Suggested incubation condition, 1hr at 370C/40C overnight
ELISA: Suggested working dilution 1/1000-1/5000. Suggested incubations conditions, 1hr at 370C
Dot blot: Use at an assay dependent concentration

This antibody is conformationally specific to native human IgG1

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