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Anti-CaMPARI-Red [4F6] antibody
RECENTLY UPDATED

Invented by Dr. Eric Schreiter at Howard Hughes Medical Institute

Info

Catalogue Number 154043
Applications IHC WB
Antigen/Gene or Protein Targets
RECENTLY UPDATED
CaMPARI1 and CaMPARI2
Reactivity N/A
Relevance
RECENTLY UPDATED
CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. CaMPARI 2.0 is the second generation of CaMPARI molecules with improve green brightness by 50% and red brightness by 250%. Additionally, CaMPARI 2.0 has faster kinetics, and a lower photoconversion rate in low calcium conditions resulting in a net 100-fold difference in green-to-red switching in low- vs. high-calcium conditions. Lastly, these different CaMPARI proteins display a range of sensitivity in their calcium binding (Kd’s ranging from 100 nM to 1 uM of free calcium).
Host
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Mouse
Immunogen
RECENTLY UPDATED
Purified and photoconverted EosFP protein, subjected it to proteolysis, and further purified the proteolytic fragment containing the red chromophore which is identical to the equivalent region of CaMPARI1 and CaMPARI2.
Subclass
RECENTLY UPDATED
IgG2a
Research Area
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Fluorescent Cell Imaging, Neurobiology

References

There are 1 reference entries for this reagent.

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References: 1 entry
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Moeyaert et al. 2018. Nat Commun. 9(1):4440. PMID: 30361563.


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References: 1 entry
RECENTLY UPDATED

Moeyaert et al. 2018. Nat Commun. 9(1):4440. PMID: 30361563.


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Inventor Information

Inventors
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Dr. Eric Schreiter

Dr. Eric Schreiter

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