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Catalogue Number
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154043 |
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Applications
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IHC
WB
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Antigen/Gene or Protein Targets
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CaMPARI1 and CaMPARI2
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Reactivity
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N/A
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Relevance
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CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. CaMPARI 2.0 is the second generation of CaMPARI molecules with improve green brightness by 50% and red brightness by 250%. Additionally, CaMPARI 2.0 has faster kinetics, and a lower photoconversion rate in low calcium conditions resulting in a net 100-fold difference in green-to-red switching in low- vs. high-calcium conditions. Lastly, these different CaMPARI proteins display a range of sensitivity in their calcium binding (Kd’s ranging from 100 nM to 1 uM of free calcium).
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Host
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Mouse
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Immunogen
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Purified and photoconverted EosFP protein, subjected it to proteolysis, and further purified the proteolytic fragment containing the red chromophore which is identical to the equivalent region of CaMPARI1 and CaMPARI2.
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Subclass
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IgG2a
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Research Area
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Fluorescent Cell Imaging, Neurobiology
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