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Catalogue Number 152721
Backbone Size (bp) 5169
Bacterial Resistance Ampicillin
Vector Type pBabe-puro
Antigen/Gene or Protein Targets Rock1, Rock
Relevance Rock1:ER Plasmid contains human ROCK I (2–537) fused to the hormone-binding domain of the estrogen receptor. The ER fusion produces a ROCK kinase that can be stimulated by estrogen analogues such as tamoxifen or 4-hydroxytamoxifen (4-HT). This stimulation can be blocked by the ROCK inhibitor Y-27632. This conditional activity is demonstrated in Croft et al., 2005. The protein is fused to eGFP for visualisation.

Ultimately, Rock1 activation leads to phosphorylation of proteins involved in the regulation of the actin‐myosin cytoskeleton, generation of pronounced actin stress fibers, and changes in cell morphology. Rock1:ER GFP serves as a good complement for small‐molecule Rock1 inhibitors.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility

References: 1 entry

Croft et al. 2005. J Cell Biol. 168(2):245-55. PMID: 15657395.


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References: 1 entry

Croft et al. 2005. J Cell Biol. 168(2):245-55. PMID: 15657395.


Add a reference

References: 1 entry

Croft et al. 2005. J Cell Biol. 168(2):245-55. PMID: 15657395.


Add a reference


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