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A2780cis Cell Line

Info

Catalogue Number 152708
Antigen/Gene or Protein Targets Cisplatin resistance
Parental Line A2780
Host Human
Tissue Ovary
Disease Keywords Human ovarian carcinoma
Model Tumour line
Relevance The cisplatin-resistant A2780cis cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (catalogue no. 152706) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media for every passage. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (catalogue no. 152707), has been isolated from the same parental line
Production Details Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 1x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherant
Recommended Growing Conditions RPMI 1640 + 2mM Glutamine + 1µM cisplatinum + 10% Foetal Bovine Serum (FBS) .
Notes Karyotype: Modal no. 46

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,13
D5S818: 11
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16
Cellosaurus ID CVCL_1942

References

There are 6 reference entries for this reagent.

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References: 6 entries

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.

Europe PMC ID: 25230021

Masuda et al. 1988. Cancer Res. 48(20):5713-6. PMID: 3139281.

Increased DNA repair as a mechanism of acquired resistance to cis-diamminedichloroplatinum (II) in human ovarian cancer cell lines.

Europe PMC ID: 3139281

Behrens et al. 1987. Cancer Res. 47(2):414-8. PMID: 3539322.

Characterization of a cis-diamminedichloroplatinum(II)-resistant human ovarian cancer cell line and its use in evaluation of platinum analogues.

Europe PMC ID: 3539322


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References: 6 entries

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.

Masuda et al. 1988. Cancer Res. 48(20):5713-6. PMID: 3139281.

Increased DNA repair as a mechanism of acquired resistance to cis-diamminedichloroplatinum (II) in human ovarian cancer cell lines.

Behrens et al. 1987. Cancer Res. 47(2):414-8. PMID: 3539322.

Characterization of a cis-diamminedichloroplatinum(II)-resistant human ovarian cancer cell line and its use in evaluation of platinum analogues.


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