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A2780ADR Cell Line

Info

Catalogue Number 152707
Antigen/Gene or Protein Targets Adriamycin resistance
Parental Line A2780
Host Human
Tissue Ovary
Disease Keywords Carcinoma
Model Tumour line
Relevance The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (catalogue no. 152706) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis (catalogue no. 152708_ these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.
Production Details Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
Notes STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
Cellosaurus ID CVCL_1941

References

There are 4 reference entries for this reagent.

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References: 4 entries

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.

Europe PMC ID: 25230021

Hamilton et al. 1984. Semin Oncol. 11(3):285-98. PMID: 6385258.

Experimental model systems of ovarian cancer: applications to the design and evaluation of new treatment approaches.

Europe PMC ID: 6385258


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References: 4 entries

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.

Hamilton et al. 1984. Semin Oncol. 11(3):285-98. PMID: 6385258.

Experimental model systems of ovarian cancer: applications to the design and evaluation of new treatment approaches.


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