Log in Register
Menu

Anti-GAL4 [2GV-3]

Info

Applications ELISA IF WB
Antigen/Gene or Protein Targets  Gal-4 (DNA Binding domain) 
Reactivity N/A
Relevance The Monoclonal Anti-Gal 4 (DNA Binding Domain), clone 2GV-3 recognises DBD containing proteins and has been validated for ELISA, Immunoblotting, Transcription assays, immunocytochemistry on transfected cells and supershift experiments. GAL4 is a modular protein consisting broadly of a DNA-binding domain and an activation domain. Although GAL4 is a yeast protein not normally present in other organisms it has been shown to work as a transcription activator in a variety of organisms such as Drosophila,[5] and human cells, highlighting that the same mechanisms for gene expression have been conserved over the course of evolution. Together with UAS (Upstream Activation Sequence), an enhancer to which GAL4 specifically binds to activate gene transcription, Gal 4 forms The GAL4-UAS system, a biochemical method used to study gene expression and function in different organisms.
Host Mouse
Immunogen Gal 4 DBD fusion protein from aa 1 to aa 147
Subclass IgG1 kappa
Notes Available in 100ul unit as ascites.

Recommended dilutions : 1/1000-1/5000

Epitope located in the second half of the DBD from aa 74 to 147
Research Area Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Epitope Tags, Metabolism

References: 1 entry

White et al. 1992. EMBO J. 11(6):2229-40. PMID: 1376247.


Add a reference

References: 1 entry

White et al. 1992. EMBO J. 11(6):2229-40. PMID: 1376247.


Add a reference

References: 1 entry

White et al. 1992. EMBO J. 11(6):2229-40. PMID: 1376247.


Add a reference


This reagent does not have any reviews at the moment.