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Monoclonal Antibodies

Anti-c-Met [12.1]

Invented by Dr Julin Wong from A*STAR Accelerate Technologies Pte Ltd , David Lane from Karolinska Institutet
Invented at University of Dundee
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Detection of endogenous c-Met in SNU-5 (c-Met positive) and T47D (c-Met negative) cells by immunofluorescence using purified anti-alpha-chain monoclonal antibodies.
Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.
Western blotting using HRP-conjugated mAb 12.1. SNU5 cell lysate was used for immunoprecipitation. SC-10 (rabbit polyclonal) is a control.
FACS analysis on live SNU-5 cells using anti-c-Met [12.1]
ELISA using anti-c-Met [12.1]
Epitope position on molecule
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Catalogue Number 152153
Applications IF IP WB
Antigen/Gene or Protein Targets c-Met, cMet
Reactivity Human
Relevance c-Met is a tyrosine receptor kinase which is activated by its ligand, the hepatocyte growth factor. Activation of c-Met leads to a wide spectrum of biological activities such as motility, angiogenesis, morphogenesis, cell survival and cell regeneration. c-Met is abnormally activated in many tumour types. Aberrant c-Met activation was found to induce tumour development, tumour cell migration and invasion, and the worst and final step in cancer progression, metastasis.
Host Mouse
Immunogen Bacterially expressed human c-Met alpha chain
Positive Control SNU-5, U-87MG and MKN45 cells (negative control: T47D cells)
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM containing 10% FCS
Strain Balb/c
Notes This clone is also referred to as 5E7/G7.

Clone 12.1 recognises a denatured epitope. The amino acid sequence of this epitope being: LEHPDCFPCQDCSSK

Does not work in FACS
Research Area Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling

References

There are 2 reference entries for this reagent.

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References: 2 entries

Wong et al. 2013. Oncotarget. 4(7):1019-36. PMID: 23859937.

IF IP WB

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Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth.

Europe PMC ID: 23859937

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References: 2 entries

Wong et al. 2013. Oncotarget. 4(7):1019-36. PMID: 23859937.

IF IP WB

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Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth.

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Add a reference

Inventor Information