Anti-CLEC2 [AYP1]

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References (2)

Inventor Info

Info

Catalogue Number	151913
Applications	FACS IF IP Fn
Antigen/Gene or Protein Targets	C-type lectin-like receptor 2, CLEC1b
Reactivity	Human
Relevance	Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.
Host	Mouse
Immunogen	A recombinant extracellular fragment of human CLEC-2 (aa 68-229), which is the extracellular domain of the protein
Positive Control	Platelets
Subclass	IgG1
Molecular Weight (kDa)	26
Myeloma Used	Sp2/0-Ag14
Recommended Growing Conditions	Hybridomas were cultured at 37º C and 96% relative humidity in air containing 6% CO2. Cells were grown in complete DMEM + Pen/strep + FCS was used at 10%. Cell density: Cells should be kept between 1-2x105 cells/ml and 5x105 cells/ml.
Notes	AYP1 can block the interaction of human CLEC-2 with its endogenous ligand Podoplanin. AYP1 does not cross react with mouse CLEC-2 when tested via FACS. AYP1 does not detect CLEC-2 by western blot. For WB we recommend clone AYP2.
Research Area	Cardiovascular, Immunology

There are 2 reference entries for this reagent.

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Gitz et al. 2014. Blood. 124(14):2262-70. PMID: 25150298.

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CLEC-2 expression is maintained on activated platelets and on platelet microparticles.

Europe PMC ID: 25150298

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Gitz et al. 2014. Blood. 124(14):2262-70. PMID: 25150298.

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CLEC-2 expression is maintained on activated platelets and on platelet microparticles.

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	University Of Birmingham
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