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Anti-CLEC2 [AYP1]

Info

Catalogue Number 151913
Applications FACS IF IP Fn
Antigen/Gene or Protein Targets C-type lectin-like receptor 2, CLEC1b
Reactivity Human
Relevance Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.
Host Mouse
Immunogen A recombinant extracellular fragment of human CLEC-2 (aa 68-229), which is the extracellular domain of the protein
Subclass IgG1
Positive Control Platelets
Molecular Weight (kDa) 26
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions Hybridomas were cultured at 37ยบ C and 96% relative humidity in air containing 6% CO2. Cells were grown in complete DMEM + Pen/strep + FCS was used at 10%. Cell density: Cells should be kept between 1-2x105 cells/ml and 5x105 cells/ml.
Notes AYP1 can block the interaction of human CLEC-2 with its endogenous ligand Podoplanin.

AYP1 does not cross react with mouse CLEC-2 when tested via FACS.

AYP1 does not detect CLEC-2 by western blot. For WB we recommend clone AYP2.
Research Area Cardiovascular, Immunology

References: 1 entry

Gitz et al. 2014. Blood. 124(14):2262-70. PMID: 25150298.


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References: 1 entry

Gitz et al. 2014. Blood. 124(14):2262-70. PMID: 25150298.


Add a reference

References: 1 entry

Gitz et al. 2014. Blood. 124(14):2262-70. PMID: 25150298.


Add a reference


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