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Monoclonal Antibodies

Anti-CLEC2 [17D9]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Specificity of anti-mouse CLEC-2 monoclonal antibody 17D9 for FACS.
Usage of 17D9 for inducing CLEC-2 signalling: LK cells ectopically expressing wild-type mouse CLEC-2 (WT) or the intracellular tyrosine mutant (Y7F) were stimulated with17D9
Usage of 17D9 for inducing CLEC-2 signalling: Lysates of BMDC stimulated with 17D9 or isotype matched mAb of irrelevant specificity were analysed by western blot using anti-phospho-Syk and anti-Syk antibodies
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Catalogue Number 151581
Applications FACS WB
Antigen/Gene or Protein Targets Ctype lectin-like receptor (CLEC2)
Reactivity Mouse
Relevance Monoclonal antibody used to study the expression pattern of CLEC-2 receptor.

Background and Research Application
CLEC-2 belongs to a sub-family of C-type lectin receptors that signal through Syk via a single YxxL motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower level on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. CLEC-2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC-2 also functions as an activation receptor on monocytes and neutrophils to induce phagocytosis and proinflammatory cytokine production.
This antibody can be used as a selective agonist of CLEC-2 and also to study its expression pattern.
Host Rat
Immunogen RBL-2H3 cells stably expressing HA-tagged mouse CLEC-2
Immunogen UniProt ID Q9P126
Subclass IgG2b
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions RPMI + 10% FCS, 2-Mercaptoethanol and antibiotics
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The first ligand that was identified for CLEC-2 was the snake venom toxin rhodocytin.

Recommended Concentrations: 10ug/ml for FACS, 50ug/ml for agonistic activity, 2ug/ml for Western.
FACS data (see figure): : B3Z cells expressing mCLEC-2-HA-IRES-GFP were mixed with parental B3Z cells (GFP negatives) and stained either with anti-HA or anti-CLEC-2-biotinylated (17D9)(5µg/ml) followed by Streptavidin-PE.

Concentration
1mg/ml as standard
Research Area Cardiovascular, Immunology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Kerrigan et al. 2009. J Immunol. 182(7):4150-7. PMID: 19299712.

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CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.

Europe PMC ID: 19299712

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References: 2 entries

Kerrigan et al. 2009. J Immunol. 182(7):4150-7. PMID: 19299712.

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CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.

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Inventor Information