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|Antigen/Gene or Protein Targets||Ctype lectin-like receptor (CLEC2)|
|Relevance||CLEC-2 belongs to a sub-family of C-type lectin receptors that signal through Syk via a single YxxL motif (hemITAM). This antibody can be used as a selective agonist of CLEC-2 and also to study its expression pattern.|
|Immunogen||RBL-2H3 cells stably expressing HA-tagged mouse CLEC-2|
|Recommended Growing Conditions||RPMI + 10% FCS, 2-Mercaptoethanol and antibiotics|
Recommended Concentrations: 10ug/ml for FACS, 50ug/ml for agonistic activity, 2ug/ml for Western.
FACS data (see figure): : B3Z cells expressing mCLEC-2-HA-IRES-GFP were mixed with parental B3Z cells (GFP negatives) and stained either with anti-HA or anti-CLEC-2-biotinylated (17D9)(5µg/ml) followed by Streptavidin-PE.
|Research Area||Cardiovascular, Immunology|
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