- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||Ctype lectin-like receptor (CLEC2)|
Monoclonal antibody used to study the expression pattern of CLEC-2 receptor.
Background and Research Application
CLEC-2 belongs to a sub-family of C-type lectin receptors that signal through Syk via a single YxxL motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower level on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. CLEC-2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC-2 also functions as an activation receptor on monocytes and neutrophils to induce phagocytosis and proinflammatory cytokine production.
This antibody can be used as a selective agonist of CLEC-2 and also to study its expression pattern.
|Immunogen||RBL-2H3 cells stably expressing HA-tagged mouse CLEC-2|
|Immunogen UniProt ID||Q9P126|
|Recommended Growing Conditions||RPMI + 10% FCS, 2-Mercaptoethanol and antibiotics|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
The first ligand that was identified for CLEC-2 was the snake venom toxin rhodocytin.
Recommended Concentrations: 10ug/ml for FACS, 50ug/ml for agonistic activity, 2ug/ml for Western.
FACS data (see figure): : B3Z cells expressing mCLEC-2-HA-IRES-GFP were mixed with parental B3Z cells (GFP negatives) and stained either with anti-HA or anti-CLEC-2-biotinylated (17D9)(5µg/ml) followed by Streptavidin-PE.
1mg/ml as standard
|Research Area||Cardiovascular, Immunology|
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