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Anti-CLEC2 [17D9]


Catalogue Number 151581
Applications FACS WB
Antigen/Gene or Protein Targets Ctype lectin-like receptor (CLEC2)
Reactivity Mouse
Relevance CLEC-2 belongs to a sub-family of C-type lectin receptors that signal through Syk via a single YxxL motif (hemITAM). This antibody can be used as a selective agonist of CLEC-2 and also to study its expression pattern.
Host Rat
Immunogen RBL-2H3 cells stably expressing HA-tagged mouse CLEC-2
Subclass IgG2b
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions RPMI + 10% FCS, 2-Mercaptoethanol and antibiotics
Notes Recommended Concentrations: 10ug/ml for FACS, 50ug/ml for agonistic activity, 2ug/ml for Western.
FACS data (see figure): : B3Z cells expressing mCLEC-2-HA-IRES-GFP were mixed with parental B3Z cells (GFP negatives) and stained either with anti-HA or anti-CLEC-2-biotinylated (17D9)(5µg/ml) followed by Streptavidin-PE.
Research Area Cardiovascular, Immunology

References: 1 entry

Kerrigan et al. 2009. J Immunol. 182(7):4150-7. PMID: 19299712.

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