Anti-ATRX [23c]
Invented by Helen Turley at University of Oxford
- Datasheet
- References (9)
- Inventor Info
Info
| Catalogue Number | 151888 |
| Applications | IHC IF WB |
| Antigen/Gene or Protein Targets | ATRX |
| Synonyms | RAD54, XNP |
| Reactivity | Human and Mouse |
| Relevance |
Monoclonal antibody which binds N terminus of human ATRX. Background and Research Application ATRX is a transcriptional regulator which is required for deposition of the histone variant H3.3 at telomeres and other genomic repeats. This is important to maintain silencing at these sites. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome ATRX is commonly mutated in cancers which maintain their telomeres by a telomerase independent pathway. This generates alternative lengthening of telomeres. This antibody binds to an epitope between amino acid residues 85-319 in the N-terminal half of human ATRX. |
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Immunogen UniProt ID | P46100 |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + HAT |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest ATRX localisation, during interphase and in mitosis, was examined using this anti-ATRX antibody. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References: 9 entries
Marano et al. 2019. Int J Mol Sci. 20(21):. PMID: 31671722.
Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.
IF
Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.
Europe PMC ID: 27892467
Mitson et al. 2011. Hum Mol Genet. 20(13):2603-10. PMID: 21505078.
WB
Functional significance of mutations in the Snf2 domain of ATRX.
Europe PMC ID: 21505078
Lukashchuk et al. 2008. J Virol. 82(24):12543-54. PMID: 18922870.
WB
Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.
Europe PMC ID: 18922870
McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.
IHC WB
Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Europe PMC ID: 10570185
Add a reference
References: 9 entries
Marano et al. 2019. Int J Mol Sci. 20(21):. PMID: 31671722.
Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.
IF
Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.
Mitson et al. 2011. Hum Mol Genet. 20(13):2603-10. PMID: 21505078.
WB
Functional significance of mutations in the Snf2 domain of ATRX.
Lukashchuk et al. 2008. J Virol. 82(24):12543-54. PMID: 18922870.
WB
Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.
McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.
IHC WB
Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Add a reference
