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Anti-ATRX [39f]
RECENTLY UPDATED

Invented by Helen Turley at University of Oxford

Info

Catalogue Number 151888
Applications
RECENTLY UPDATED
IHC IF WB
Antigen/Gene or Protein Targets ATRX
Synonyms RAD54, XNP
Reactivity Human and Mouse
Relevance
RECENTLY UPDATED
Monoclonal antibody which binds N terminus of human ATRX.

Background and Research Application
ATRX is a transcriptional regulator which is required for deposition of the histone variant H3.3 at telomeres and other genomic repeats. This is important to maintain silencing at these sites. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome
ATRX is commonly mutated in cancers which maintain their telomeres by a telomerase independent pathway. This generates alternative lengthening of telomeres.

This antibody binds to an epitope between amino acid residues 85-319 in the N-terminal half of human ATRX.
Host Mouse
Immunogen Recombinant Protein
Immunogen UniProt ID
RECENTLY UPDATED
P46100
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + HAT
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
ATRX localisation, during interphase and in mitosis, was examined using this anti-ATRX antibody.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Cycle, Epigenetics & Nuclear Signalling

References

There are 9 reference entries for this reagent.

View All References

References: 9 entries
RECENTLY UPDATED

Marano et al. 2019. Int J Mol Sci. 20(21):. PMID: 31671722.

Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.

IF

Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.

Europe PMC ID: 27892467

Mitson et al. 2011. Hum Mol Genet. 20(13):2603-10. PMID: 21505078.

WB

Functional significance of mutations in the Snf2 domain of ATRX.

Europe PMC ID: 21505078

Lukashchuk et al. 2008. J Virol. 82(24):12543-54. PMID: 18922870.

WB

Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.

Europe PMC ID: 18922870

McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.

IHC WB

Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.

Europe PMC ID: 10570185


Add a reference

References: 9 entries
RECENTLY UPDATED

Marano et al. 2019. Int J Mol Sci. 20(21):. PMID: 31671722.

Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.

IF

Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.

Mitson et al. 2011. Hum Mol Genet. 20(13):2603-10. PMID: 21505078.

WB

Functional significance of mutations in the Snf2 domain of ATRX.

Lukashchuk et al. 2008. J Virol. 82(24):12543-54. PMID: 18922870.

WB

Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.

McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.

IHC WB

Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.


Add a reference