Anti-ATRX [39f]
Invented by Helen Turley at University of Oxford
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151887 |
| Applications | IHC IF WB |
| Antigen/Gene or Protein Targets | ATRX |
| Synonyms | RAD54, XNP |
| Reactivity | Human and Mouse |
| Relevance |
Monoclonal antibody which detects a commonly mutated telomere regulator. Background and Research Application ATRX is a transcriptional regulator which is required for deposition of the histone variant H3.3 at telomeres and other genomic repeats. This is important to maintain silencing at these sites. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome ATRX is commonly mutated in cancers which maintain their telomeres by a telomerase independent pathway. This generates alternative lengthening of telomeres. |
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Immunogen UniProt ID | P46100 |
| Positive Control | HeLa and L929 cells |
| Subclass | IgG3 kappa |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + HAT |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest ATRX localisation, during interphase and in mitosis, was examined using this anti-ATRX antibody. Staining Pattern: nuclear Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References: 4 entries
Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.
IF
Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.
Europe PMC ID: 27892467
McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.
WB IF
Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Europe PMC ID: 10570185
Add a reference
References: 4 entries
Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.
IF
Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.
McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.
WB IF
Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Add a reference
