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Anti-ATRX [23c]

Invented by Helen Turley at University of Oxford

Info

Catalogue Number 151887
Applications IHC IF WB
Antigen/Gene or Protein Targets ATRX
Synonyms RAD54, XNP
Reactivity Human and Mouse
Relevance Monoclonal antibody which detects a commonly mutated telomere regulator.

Background and Research Application
ATRX is a transcriptional regulator which is required for deposition of the histone variant H3.3 at telomeres and other genomic repeats. This is important to maintain silencing at these sites. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome
ATRX is commonly mutated in cancers which maintain their telomeres by a telomerase independent pathway. This generates alternative lengthening of telomeres.
Host Mouse
Immunogen Recombinant Protein
Immunogen UniProt ID P46100
Positive Control HeLa and L929 cells
Subclass IgG3 kappa
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + HAT
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
ATRX localisation, during interphase and in mitosis, was examined using this anti-ATRX antibody.
Staining Pattern: nuclear

Concentration
1mg/ml as standard
Research Area Cancer, Cell Cycle, Epigenetics & Nuclear Signalling

References

There are 4 reference entries for this reagent.

View All References

References: 4 entries

Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.

IF

Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.

Europe PMC ID: 27892467

McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.

WB IF

Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.

Europe PMC ID: 10570185


Add a reference

References: 4 entries

Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.

IF

Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.

McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.

WB IF

Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.


Add a reference