Cat. #151888
Anti-ATRX [39f]
Cat. #: 151888
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: ATRX
Class: Monoclonal
Application: IHC ; IF ; WB
Reactivity: Human ; Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Helen Turley
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-ATRX [39f]
- Alternate name: RAD54, XNP
- Clone: 39f
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human ; Mouse
- Host: Mouse
- Application: IHC ; IF ; WB
- Description: Monoclonal antibody which binds N terminus of human ATRX.
- Immunogen: Recombinant Protein
- Immunogen uniprot id: P46100
- Isotype: IgG1
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: ATRX
- Target background: ATRX is a transcriptional regulator which is required for deposition of the histone variant H3.3 at telomeres and other genomic repeats. This is important to maintain silencing at these sites. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome ATRX is commonly mutated in cancers which maintain their telomeres by a telomerase independent pathway. This generates alternative lengthening of telomeres. This antibody binds to an epitope between amino acid residues 85-319 in the N-terminal half of human ATRX.
Applications
- Application: IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-ATRX [23c]
References
- Marano et al. 2019. Int J Mol Sci. 20(21):. PMID: 31671722.
- Cooper et al. 2016. Nat Commun. 7:13661. PMID: 27892467.
- Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.
- Mitson et al. 2011. Hum Mol Genet. 20(13):2603-10. PMID: 21505078.
- Fn significance of mutations in the Snf2 domain of ATRX.
- Lukashchuk et al. 2008. J Virol. 82(24):12543-54. PMID: 18922870.
- Human cytomegalovirus protein pp71 displaces the chromatin-associated factor ATRX from nuclear domain 10 at early stages of infection.
- McDowell et al. 1999. Proc Natl Acad Sci U S A. 96(24):13983-8. PMID: 10570185.
- Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
