Cancer Research Technology
Log in Register
Menu
Search
Monoclonal Antibodies

Anti-Talin1 [93E12]

Invented at University Of Leicester
  • Datasheet
  • Inventor Info
Print

Info

Image thumbnail for Anti-Talin1 [93E12]
Image thumbnail for Anti-Talin1 [93E12]
Image thumbnail for Anti-Talin1 [93E12]
Add an image
Adapted from Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379. Figure. (B) Purified recombinant talin rod polypeptides (50 ng) spanning residues 489–911 (upper panel) or 2300–2542 (lower panel) were resolved by SDS-PAGE and either stained with Coomassie blue (coo) or analysed by Western blotting using talin Mabs 97H6, 93E12, 68E7, 121A as well as the commercial talin Mab 8d4 (Sigma). (C) The epitopes recognised by Mab 93E12 and 97H6 were further defined using recombinant talin1 rod polypeptides containing either residues 482–655 or residues 656–911. Mab 97H6 recognises the former while 93E12 recognises the latter. (D) Western blots of whole cell lysates from untransfected mouse embryo fibroblasts (40 μg protein) or lysates from cells expressing either GFP-talin1, GFP-talin2 or GFP alone (2.5 μg protein) probed with the talin Mabs indicated.
Adapted from Praekelt et al. 2012. Eur J Cell Biol; 91(3):180-91. PMID: 22306379. Figure. Immuno-staining of paraffin-embedded sections of wild type and talin2 null mouse kidney with antibodies to talin1 (93E12). Staining is present in the glomerulus, tubules and interstitium in the cortex, and the tubules and interstitium in the medulla
Adapted from Chen et al. 2017. Oncotarget; 8(8):13003-13014. PMID: 28099903. Figure (B) Western blot analysis of Talin-1 protein expression. Talin-1 is more highly expressed in MHCC-97L cells compared with LO2 cells in terms of protein amounts.
Add an image
Image thumbnail for Anti-Talin1 [93E12] Image thumbnail for Anti-Talin1 [93E12] Image thumbnail for Anti-Talin1 [93E12]
Catalogue Number 151659
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets Talin 1
Reactivity Mouse
Relevance Talin 1 is a high molecular weight protein found in a variety of tissues and cell types. Talin 1 is essential for integrin-mediated cell adhesion. This antibody can be used for specific detection of the focal adhesion protein talin 1 and does not cross react with talin 2.
Host Mouse
Immunogen Recombinant protein fragment
Positive Control fibroblasts
Subclass IgG1
Myeloma Used NS0
Recommended Growing Conditions RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2
Notes Suggested dilutions: 1/100 - 1/1000
Research Area Adhesion, Cell Signaling & Signal Transduction

References

There are 6 reference entries for this reagent.

View All References

References: 6 entries

Chen et al. 2017. Oncotarget. 8(8):13003-13014. PMID: 28099903.

WB

View  

van de Klundert et al. 2016. Viruses. 8(10):. PMID: 27775586.

WB

View  

Kumar et al. 2016. J Cell Biol. 213(3):371-83. PMID: 27161398.

WB

View  

Liu et al. 2015. Proc Natl Acad Sci U S A. 112(35):E4864-73. PMID: 26283369.

WB

View  

Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379.

IHC WB

View  

New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.

Europe PMC ID: 22306379

View  

Add a reference

References: 6 entries

Chen et al. 2017. Oncotarget. 8(8):13003-13014. PMID: 28099903.

WB

View  

van de Klundert et al. 2016. Viruses. 8(10):. PMID: 27775586.

WB

View  

Kumar et al. 2016. J Cell Biol. 213(3):371-83. PMID: 27161398.

WB

View  

Liu et al. 2015. Proc Natl Acad Sci U S A. 112(35):E4864-73. PMID: 26283369.

WB

View  

Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379.

IHC WB

View  

New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.

View  

Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor

Invented at

University Of Leicester
University Of Leicester

20 reagents listed on Ximbio