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Anti-Talin1 [97H6]

Info

Catalogue Number 151627
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets Talin 1
Reactivity Human
Relevance Monoclonal antibody for investigation into cell adhesion with no cross reactivity with related talin 2 protein.

Background and Research Application
Talin 1 is a high molecular weight protein found in a variety of tissues and cell types. Talin 1 is essential for integrin-mediated cell adhesion, the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. Talin 1 also links lymphocytes at cell-cell contact points. The N-terminus of talin 1 contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
This antibody recognizes an epitope within the region of amino acids 482-655 of human talin-1. Anti-talin1 antibody can be used for specific detection of the focal adhesion protein talin 1.
Host Mouse
Immunogen His-tagged polypeptide containing residues 489-911 of mouse Talin1
Immunogen UniProt ID Q9Y490
Positive Control Fibroblasts
Subclass IgG1
Myeloma Used NS0
Recommended Growing Conditions RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Suggested dilutions: 1/100 - 1/1000
Anti-Talin1 antibody does not cross react with talin 2.

Concentration
1mg/ml as standard
Research Area Adhesion, Cell Signaling & Signal Transduction

References

There are 12 reference entries for this reagent.

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References: 12 entries

Li et al. 2017. Oncotarget. 8(63):106876-106887. PMID: 29290996.

Bouchet et al. 2016. Elife. 5:. PMID: 27410476.

Debrand et al. 2012. Biochem Biophys Res Commun. 426(2):190-5. PMID: 22925892.

IHC WB

Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.

Europe PMC ID: 22925892

Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379.

New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.

Europe PMC ID: 22306379

Liu et al. 2011. Mol Cell Biol. 31(16):3366-77. PMID: 21670148.

WB

Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.

Europe PMC ID: 21670148

Monkley et al. 2011. Dev Biol. 349(2):494-502. PMID: 21081121.

IHC WB

Endothelial cell talin1 is essential for embryonic angiogenesis.

Europe PMC ID: 21081121

Kopp et al. 2010. Eur J Cell Biol. 89(9):661-73. PMID: 20605055.

IF

Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.

Europe PMC ID: 20605055


Add a reference

References: 12 entries

Li et al. 2017. Oncotarget. 8(63):106876-106887. PMID: 29290996.

Bouchet et al. 2016. Elife. 5:. PMID: 27410476.

Debrand et al. 2012. Biochem Biophys Res Commun. 426(2):190-5. PMID: 22925892.

IHC WB

Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.

Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379.

New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.

Liu et al. 2011. Mol Cell Biol. 31(16):3366-77. PMID: 21670148.

WB

Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.

Monkley et al. 2011. Dev Biol. 349(2):494-502. PMID: 21081121.

IHC WB

Endothelial cell talin1 is essential for embryonic angiogenesis.

Kopp et al. 2010. Eur J Cell Biol. 89(9):661-73. PMID: 20605055.

IF

Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

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