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Monoclonal Antibodies

Anti-Talin2 [68E7]
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Adapted from Debrand et al. 2009. FEBS J. 276(6):1610-28. PMID: 19220457. Figure. Proteins from wild-type (wt) and homozygous gene trap (gt/gt) mice were analysed by western blotting. Equal amount of total proteins were loaded, and talin 2 was detected with a monoclonal antibody against talin 2 (epitope within residues 482–991). Vinculin was used as an additional loading control.
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RECENTLY UPDATED
Adapted from Debrand et al. 2009. FEBS J. 276(6):1610-28. PMID: 19220457. Figure. Western blotting of testis protein extracts from two C57Bl/6 mice prior to (2 weeks old) and after puberty (4, 8 and 12 weeks). A talin 2 monoclonal CN-terminal monoclonal antibody detected only full-length talin 2. Vinculin was used as a loading control
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RECENTLY UPDATED
Adapted from Bouchet et al. 2016. Elife. 5. PMID: 27410476. Figure. Western blot analysis of talin1/talin2 co-depletion by independent siRNAs (KANK1#1, K1 #1, KANK1#2, K1 #2 and KANK2#1, K2 #1; talin1 #2, t1 #2 and talin2#1, t2 #1) compared to control siRNA (CT). Ku80, loading control.
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Adapted from Debrand et al. 2009. FEBS J. 276(6):1610-28. PMID: 19220457. Figure. Proteins from wild-type (wt) and homozygous gene trap (gt/gt) mice were analysed by western blotting. Equal amount of total proteins were loaded, and talin 2 was detected with a monoclonal antibody against talin 2 (epitope within residues 482–991). Vinculin was used as an additional loading control.
Adapted from Debrand et al. 2009. FEBS J. 276(6):1610-28. PMID: 19220457. Figure. Western blotting of testis protein extracts from two C57Bl/6 mice prior to (2 weeks old) and after puberty (4, 8 and 12 weeks). A talin 2 monoclonal CN-terminal monoclonal antibody detected only full-length talin 2. Vinculin was used as a loading control
Adapted from Bouchet et al. 2016. Elife. 5. PMID: 27410476. Figure. Western blot analysis of talin1/talin2 co-depletion by independent siRNAs (KANK1#1, K1 #1, KANK1#2, K1 #2 and KANK2#1, K2 #1; talin1 #2, t1 #2 and talin2#1, t2 #1) compared to control siRNA (CT). Ku80, loading control.
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RECENTLY UPDATED
Catalogue Number 151658
Applications
RECENTLY UPDATED
 ELISA IHC IF IP WB
Antigen/Gene or Protein Targets Talin 2
Reactivity Mouse
Relevance Talin 2 is a high molecular weight protein found in a variety of tissues and cell types. Talin 2 interacts with integrins but it's full functions are not known. This antibody can be used for the specific detection of the focal adhesion protein talin 2 and does not cross react with talin 1.
Host Mouse
Immunogen recombinant protein fragment
Positive Control fibroblasts
Subclass IgG2b
Myeloma Used NS0
Recommended Growing Conditions RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2
Notes Suggested Dilutions: 1/100 - 1/1000
Research Area Cell Signaling & Signal Transduction

References

There are 5 reference entries for this reagent.

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References: 5 entries
RECENTLY UPDATED

Böttcher et al. 2017. J Cell Biol. 216(11):3785-3798. PMID: 28912124.

WB

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Bouchet et al. 2016. Elife. 5:. PMID: 27410476.

WB

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Theodosiou et al. 2016. Elife. 5:e10130. PMID: 26821125.

WB

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Debrand et al. 2009. FEBS J. 276(6):1610-28. PMID: 19220457.

WB

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Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms.

Europe PMC ID: 19220457

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References: 5 entries
RECENTLY UPDATED

Böttcher et al. 2017. J Cell Biol. 216(11):3785-3798. PMID: 28912124.

WB

View  

Bouchet et al. 2016. Elife. 5:. PMID: 27410476.

WB

View  

Theodosiou et al. 2016. Elife. 5:e10130. PMID: 26821125.

WB

View  

Debrand et al. 2009. FEBS J. 276(6):1610-28. PMID: 19220457.

WB

View  

Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms.

View  

Add a reference

Inventor Information

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Invented at

University Of Leicester
University Of Leicester

18 reagents listed on Ximbio