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Catalogue Number 151289
Applications FACS IHC IF IP
Antigen/Gene or Protein Targets TCR Cβ1
Reactivity Human
Relevance Monoclonal antibody which aids investigation into T-cell related diseases.

Background and Research Application
The T cell receptor is expressed on all mature T cells. The T cell receptor is heterodimer of alpha/beta or gamma/delta chains, expressed on all mature T cells. TCR’s recognize antigen peptides bound to MHC molecules, providing the basis of antigen specific response by T cells. Genetic mutations involving the T cell receptor β locus have been associated with T cell lymphomas.

JOVI.1 can be used for studies of T-cell mediated diseases, including autoimmunity and allergy. Antibody JOVI·1 recognised a determinant on the majority of TCRs, staining 50-75% of peripheral blood T cells and T cell lines expressing different Vβ regions.
Host Mouse
Immunogen Human HA1.7 TCR beta chain expressed on transgenic mouse cells.
Immunogen UniProt ID P01850
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
JOVI.1 monoclonal antibody that targets TCRBC1+ cells in a selective manner by recognizing 2 amino acid residues specifically exposed in TCRBC1 but not in TCRBC2.

Concentration
1mg/ml as standard
Research Area Cell Type or Organelle Marker, Immunology

References

There are 9 reference entries for this reagent.

View All References

References: 9 entries

Rossatti et al. 2019. Cells. 8(11):. PMID: 31690048.

Beck et al. 2018. Hum Vaccin Immunother. 14(12):2864-2873. PMID: 30111232.

Tesio et al. 2018. Hemasphere. 2(2):e38. PMID: 31723766.

T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation.

Europe PMC ID: 18320063

Risueño et al. 2008. PLoS One. 3(3):e1747. PMID: 18320063.

Gil et al. 2002. Cell. 109(7):901-12. PMID: 12110186.

Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.

Europe PMC ID: 12110186

Viney et al. 1992. Hybridoma. 11(6):701-13. PMID: 1284120.

Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice.

Europe PMC ID: 1284120


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References: 9 entries

Rossatti et al. 2019. Cells. 8(11):. PMID: 31690048.

Beck et al. 2018. Hum Vaccin Immunother. 14(12):2864-2873. PMID: 30111232.

Tesio et al. 2018. Hemasphere. 2(2):e38. PMID: 31723766.

T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation.

Risueño et al. 2008. PLoS One. 3(3):e1747. PMID: 18320063.

Gil et al. 2002. Cell. 109(7):901-12. PMID: 12110186.

Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.

Viney et al. 1992. Hybridoma. 11(6):701-13. PMID: 1284120.

Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice.


Add a reference